实验性骨质疏松对骨缺损自然愈合的影响  

The effect of osteoporosis on bone defect healing without i mplanting

作　　者：段王平[1] 张民[1] 李刚 卫小春[1] 

机构地区：[1]山西医科大学第二医院骨科,太原030001 [2]英国北爱尔兰女王大学医学院

出　　处：《中国骨质疏松杂志》2008年第2期80-84,94,共6页Chinese Journal of Osteoporosis

基　　金：山西省留学归国人员基金(2006074)

摘　　要：目的探讨骨质疏松对大鼠股骨骨缺损自然修复过程的影响。方法选用8月龄雌性SD大鼠20只,随机分为两组。实验组行双侧卵巢摘除术,对照组行假手术。术后12w两组大鼠双侧股骨外侧髁上造成直径3mm骨缺损,其深度为一侧骨皮质和髓腔宽度之和。缺损术后4、8、12w取双侧股骨标本,进行组织学、X线、骨密度、透射电镜及生物力学检测。结果切除卵巢后3个月大鼠体重明显增加(P<0.01)。两组骨缺损到12w时都未能完全愈合,实验组新生骨痂少,成骨过程延缓,破骨细胞功能活跃,4、8w时实验组缺损部位X线灰度、骨密度值均明显低于对照组(P<0.05)。结论大鼠股骨髁上直径3mm缺损到12w时未能完全愈合,同时,特别是在早期,骨质疏松更加延缓了该缺损自然修复的过程。Objective To investigate the effect of osteoporosis on the healing of femur bone defects without implanting in rats. Methods Twenty female SD rats （8 months old） were randomly divided into two groups. In experimental group ovariectomy was performed and in control group sham operation was received. Twelve weeks after surgery, cylindrical defect of which the diameter is 3.0mm and depth to medial cortical bone were performed on both condylus lateralis femoris and no graft in both groups. The effect of bone defect healing was evaluated in the 4^th ,8^th and 12^th week after operation by histomorphology, radiology, bone mineral density （BMD）, transmission electron microscope （TEM） and vitodynamics. Results The body weight obviously increased in 3^rd month after ovariectomized （ P 〈 0.01 ）. In both groups, the defects cannot heal completely in 12^th week. In experimental group, new bone was fewer and the growth was delayed, meanwhile, the osteoclasts were active. Radiological gray scale and BMD of defects all significantly decreased in experimental group compared with control group in the 4^th and 8^th week （ P 〈 0.05）. Conclusions This defect cannot heal completely in 12^th week, meanwhile, especially in the early stage, the healing of bone defects can be delayed by osteoporosis.

关 键 词：骨质疏松 骨缺损 修复 

分 类 号：R683[医药卫生—骨科学] R589.5[医药卫生—外科学]