羟基磷灰石膜复合材料与人血管内皮细胞的相容性  

Study on biocompatibility of hydroxyapatite film complex materials and human blood vessel endothelial cells

作　　者：程光存[1] 严中亚[1] 罗乐[2] 方晓东[3] 沙自明[1] 

机构地区：[1]安徽医科大学附属省立医院心脏外科,合肥230001 [2]合肥工业大学理学院,合肥230009 [3]中科院安徽光学精密机械研究所,合肥230031

出　　处：《安徽医科大学学报》2007年第6期626-629,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省人才培养及科研带头人专项基金〔编号:(2005)58〕

摘　　要：目的利用脉冲激光沉积方法在人工心脏机械瓣膜上沉积纳米相羟基磷灰石(HA)薄膜,探讨其组织相容性。方法将人血管内皮细胞与纳米相HA薄膜复合材料体外复合培养,进行形态学和功能测定,同时分别测定在HA材料组和空白对照组中血管内皮细胞分泌一氧化氮(NO)、前列环素(PGI2)水平。结果人血管内皮细胞能在纳米相HA薄膜复合材料上良好地黏附、增殖、生长。人脐静脉血管内皮细胞分泌NO和PGI2在HA材料和空白对照组差异无显著性(P>0.05)。结论纳米相HA薄膜复合材料具有良好的细胞相容性,可作为组织工程化机械瓣膜材料。Objective To use impulse laser sediment（PLD） method to make nanometeric hydroxyapatite （HA）film sedimenting on the surface of artificial heart valve, in order to explore the biocompatibility of nanometeric HA film complex materials. Methods Human vascular endothelial cells and nanometerie HA film complex materials were co-cultivated in vitro. Morphological and functional indexes of human vascular endothelial cells were measured. The levels of nitric oxide （NO） and PGI2 secreted by vascular endothelial cells of HA group and normal control group were measured. Results Human vascular endothelial cells can well adhere to nanometeric HA film complex materials and could well multiply and grow. The level of NO and PGI2 secreted by human vascular endothelial cells had no significant difference （ P 〉 0. 05 ）. Conclusion HA film complex material has good biocompatibility and could be used as tissue engineering artificial mechanical valve material.

关 键 词：羟基磷灰石类 组织工程 内皮 血管 细胞培养技术 

分 类 号：R318.2[医药卫生—生物医学工程] R322.12[医药卫生—基础医学]