野菊花总黄酮对佐剂性关节炎大鼠滑膜组织中TRAIL、TNF-α表达的影响  

Effect of total flavonoids of Chrysanthemum indicum on TRAIL,TNF-α expression of synovial tissue from adjuvant arthritis rats in vivo

作　　者：解雪峰[1] 李俊[1] 陈晓宇[1] 姜辉[1] 

机构地区：[1]安徽医科大学药学院,安徽合肥230032

出　　处：《中国药理学通报》2007年第12期1662-1666,共5页Chinese Pharmacological Bulletin

基　　金：国家科技部重大基础研究前期研究资助项目(No2002CCCC02900);安徽医科大学校科研基金资助项目(No2006kj20)

摘　　要：目的研究野菊花总黄酮对佐剂性关节炎大鼠滑膜细胞的增殖功能以及滑膜组织中TRAIL、TNF-α表达的影响。方法SD大鼠随机分成6组:正常组、模型组、TFC大、中、小剂量组和阳性药雷公藤多苷(TPT)对照组;SD大鼠右后足跖皮内注射弗氏完全佐剂复制AA模型;MTT法检测滑膜细胞的增殖;运用免疫荧光法检测各组滑膜组织TRAIL、TNF-α蛋白的表达。结果TFC可剂量依赖性地降低AA大鼠滑膜细胞的炎性增殖;TRAIL在模型组大鼠滑膜组织中的表达明显低于正常组;TFC各剂量组可上调TRAIL的过低表达,而TNF-α的检测结果与之相反。结论以TNF-α为参照,TRAIL参与AA大鼠的发病过程,TFC可上调TRAIL蛋白的过低表达,这可能是其治疗AA的机制之一。Aim To investigate the effect of total flavonoids of Chrysanthemum indicum on proliferation of synoviocytes and TRAIL/TNF-α expression of synovial tissue with adjuvant arthritis rats. Methods SD rats were divided randomly into six groups including normol,model,TFC （84,168,336 mg·kg^-1 ）and control drug Tripterygium glycosides （30mg·kg^-1）groups. Adjuvant arthritis rat model was induced by a single intradermal injection of 0. 1 ml of the complete Freund＇s adjuvant into the the fight hind feet pads of the SD rats. The proliferation of synoviocyte was measured by MTT; The expression of TRAIL and TNF-α on synovial tissue were detected by means of immunofluorescence. Results TFC was shown to suppress the excessive synoviocyte proliferation;The expression of TRAIL was lower in model group than that of the normal group. TFC can increase the expression of TRAIL protein; The contrary to TNF-α protein. Conclusions TRAIL play a role in the development of AA. TFC can increase the lower expression of TRAIL protein, that maybe one of the mechanisms of TFC improvement AA.

关 键 词：野菊花总黄酮 佐剂性关节炎 肿瘤坏死因子相关凋亡诱导配体 肿瘤坏死因子α 

分 类 号：R282.71[医药卫生—中药学] R392.114[医药卫生—中医学]