高浓度葡萄糖对人脐静脉内皮细胞内皮型一氧化氮合酶活性及其蛋白表达的影响  

Effects of high concentration of glucose on activity and protein expression of endothelial nitric oxide synthase (eNOS) in cultured human umbilical vein endothelial cells(HUVECs)

作　　者：杨慎启[1] 徐标[2] 刘云海[1] 

机构地区：[1]山东济宁市第一人民医院急诊科,272011 [2]南京大学附属南京鼓楼医院心内科

出　　处：《中国糖尿病杂志》2008年第1期50-52,共3页Chinese Journal of Diabetes

基　　金：江苏省社会发展基金资助项目(BS2000032)

摘　　要：不同浓度葡萄糖及高浓度葡萄糖(高糖)加胰岛素孵育人脐静脉内皮细胞(HUVECs)不同时间后,高浓度葡萄糖呈浓度和时间依赖性明显抑制内皮型一氧化氮合酶(eNOS)活性,生理浓度的胰岛素可部分地逆转高糖对eNOS活性及其表达的抑制作用。The activity of eNOS was singificantly depressed by a high concentration of glucose in a concentration-and time-dependent manners after incubation of HUVECs with different concentrations of glucose and with high glucose plus insulin for different times. The physiological concentration of insulin can partially reverse the inhibitions of the activity and expression of eNOS induced by high concentration of glucose.

关 键 词：人脐静脉内皮细胞 一氧化氮合酶 高浓度葡萄糖 

分 类 号：R587.2[医药卫生—内分泌]