柱前衍生化法评价不同品种和产地柴胡药材和饮片的质量  

Determination of content of saikoside in Bupleurum Chinense from different sources

作　　者：李媛媛[1] 秦雪梅[1] 王玉庆[2] 岳建英[3] 郝建平[4] 

机构地区：[1]山西大学化学化工学院药学系,山西太原030006 [2]山西农业大学农学院,山西太谷030801 [3]山西省生物研究所,山西太原030006 [4]山西大学生命科学与技术学院,山西太原030006

出　　处：《中国中药杂志》2008年第3期237-240,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30570174);山西省自然科学基金项目(20051085);山西省科技攻关项目(051077-2)

摘　　要：目的：通过对大量柴胡药材和柴胡饮片样品中柴胡总皂苷和柴胡皂苷a，d的含量进行测定评价不同产地、不同品种柴胡样品的质量优劣。方法：分光光度法测定柴胡总皂苷的含量；柱前衍生化HPLC-UV测定柴胡皂苷a，d的含量。结果：红柴胡、黑柴胡的皂苷类成分较北柴胡低，竹叶柴胡几乎检不出柴胡皂苷；北柴胡的柴胡皂苷a的质量分数主要在0.100％～0．330％，柴胡皂苷d0．200％～0．400％，柴胡总皂苷1.00％～2．00％；粗根无分支柴胡皂苷成分明显低于细根或粗根多分支柴胡药材。结论：建议北柴胡的柴胡皂苷a的含量不得低于0．100％，柴胡皂苷d不得低于0．200％，柴胡总皂苷不得低于1．00％；北柴胡的适宜产区为山西中部的太谷，山西南部的芮城，平陆，万荣，山西东南部的长治，甘肃的陇西，礼县等地；选育北柴胡品种时优先选取根粗且多分支的品种。Objective： To determine the contents of saikosaponin a, d and saikosaponins in Radix Bupleuri from different and assess their quality. Method： Saikosaponin a and d were used as the chemical reference substance to establish RP-HPLC and visible- spectrophotometry methods for determination of contents of saikosaponin a, d and saikosaponins. Result： The quality of B. chinense was better than that of B. scozonerifolium and B smishii; The contents of a, saikosaponin d and total saikosaponin in B. chinense were 0. 100%-0. 330%, 0. 200%-0. 400% and 1.00%-2.00%, respectively. Conclusion： It is to suggest that the minimal contents requirements of saikosaponin a, d and saikosaponins in B. chinense should be set at 0. 100% , 0.200% and 1.00% , respectively; the best suitable planting areas are Taigu in the middle of Shanxi province, Ruicheng, Pinglu and Wanrong in the south of Shanxi province, and Longxi, Lixian in Gansu province.

关 键 词：柴胡 柴胡皂苷 分光光度法 高效液相色谱法 含量测定 

分 类 号：R284[医药卫生—中药学]