参脉注射液对实验性肺缺血／再灌注损伤时血红素氧合酶-1表达与活性的影响  

Effect of Shenmai injection on expression and activity of heme oxygenase-1 in reperfusion injury after pulmonary ischemia in rabbits

作　　者：林丽娜[1] 张圣恭[1] 王万铁[2] 习建华[1] 邱晓晓 戴雍月[2] 

机构地区：[1]温州医学院附属第一医院,浙江温州325000 [2]温州医学院病理生理教研室,浙江温州325027

出　　处：《中国中药杂志》2008年第3期296-299,共4页China Journal of Chinese Materia Medica

基　　金：浙江省中医药管理局科研基金(2005C088)

摘　　要：目的:探讨参脉注射液对兔肺缺血-再灌注损伤时血红素氧合酶-1(HO-1)表达与活性的影响。方法:采用在体兔单肺原位缺血-再灌注损伤模型。实验兔20只,随机均分为肺缺血-再灌注损伤组(I-R组)和参脉注射液治疗组(SM组,15 mL.kg-1)。用原位杂交方法观察HO-1在兔肺组织中的表达变化;测定肺组织湿干重比(W/D)及肺泡损伤数(IAR);电镜观察肺组织超微结构的改变。结果:HO-1在I-R组、SM组肺血管内皮、部分血管平滑肌、外膜层及部分气道上皮均有阳性表达,原位杂交的平均吸光度分别为0.148±0.013,0.158±0.012,SM组HO-1的表达水平明显高于I-R组(P<0.01),W/D,IAR值显著低于I-R组(P<0.01),肺组织形态学异常改变轻于I-R组。结论:参脉注射液可通过提高肺组织HO-1的表达水平,对肺缺血-再灌注损伤发挥积极的保护作用。Objective： To explore the effect of Shenmai injection the expression of heme oxygenase-1 （ HO-1 ） in rabbits with reperfusion injury after pulmonary ischemia. Method： Single lung ischemia/reperfusion injury animal model was used in vivo. Twenty rabbits were randomly divided into two groups （n = 10, in each） , pulmonary ischemia and reperfusion injury （PIRI） group and I- R ＋ Shenmai injection group. The tissue slides were stained by in situ hybridization （ISH） for HO-1 to detect the expression of HO-1 in lung and to analyze the absorbance, Wet to dry ratio of lung tissue weight （W/D） and the injured alveoli rate （IAR） were measured at 180 minutes after lung reperfusion. Meanwhile the lung tissue slide was prepared for electron microscopic observation at 180 minutes after reperfusion. Result： HO-1 expression was upregulated in two groups in the pulmonary endothelial cells, part of pulmonary vascular smooth muscle cells, extima of vessels and epithelial cells of airway, the absorbance was 0. 148 ± 0. 013, 0. 158 ± 0. 012, respectively. The Shenmai injection group showed higher absorbance than those of the IRI group （ P 〈0. 01 ）, lower W/D and IAR values than those of the IRI group （ P 〈 0. 01 ） significantly and lighter abnormal changes of the lung tissue in morphologically than those of the PIRI group. Conclusion： Shenmai injection possesses notable protective effects on PIRI in rabbits by increasing the expression of HO-1 in lung.

关 键 词：参脉注射液 再灌注损伤 肺 血红素氧合酶-1 

分 类 号：R285.5[医药卫生—中药学]