原花青素预防大鼠亚硒酸钠性白内障的机制研究  

Study on preventing effects of procyanidins on selenite cataract development in rats

作　　者：成旋[1] 程薇波[2] 颜灏 张素才[2] 王宇[2] 

机构地区：[1]成都市第六人民医院,四川成都610051 [2]四川大学华西公共卫生学院病理教研室,四川成都610041 [3]四川剑南春生物制品有限责任公司,四川成都610071

出　　处：《中国中药杂志》2008年第3期300-302,共3页China Journal of Chinese Materia Medica

基　　金：成都市卫生局青年科学研究基金(200406)

摘　　要：目的:研究原花青素(PC)对大鼠亚硒酸钠性白内障的预防作用并探讨其作用的时效关系。方法:SD大鼠45只,随机分为正常组、模型组和药物组,药物组在给予亚硒酸钠的同时给予80 mg.kg-1原花青素。给药第6,11,16天,分别放血处死每组动物的5只。测定晶状体中丙二醛(MDA)、超氧化物歧化酶(SOD)、以及谷胱甘肽过氧化物酶(GSH-Px)含量。结果:第10,15天的药物组晶状体中MDA含量有显著性降低(P<0.01),第15天的药物组晶状体中SOD,GSH-Px含量有显著性增高(P<0.01)。其作用机制与其良好的抗氧化作用有关。结论:原花青素可明显抑制大鼠晶状体白内障发生发展,而且晶状体中MDA,SOD,GSH-Px含量在本实验中呈现明显的时间效应关系,推荐给药时间是15 d。Objective： To study the preventing effects of procyanidins （PC） on selenite cataract in rats and the time-effect relationship. Method： Forty five SD rats were divided into three groups ： control, model and experiment groups. The rats in the experiment group were fed additionally with the PC by 80 mg · kg^-1 when they were supplied the equal selenite with the model group. Five rats of each group were regularly sacrificed by bleeding from femoral artery at sixth, eleventh, sixteenth day and the level of malondialdehyde （MDA）, superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） of all lenses was measured. Result： Compared with the model group, the level of the MDA in the experiment group at the eleventh day and the sixteenth day greatly decreased （P 〈 0. 01 ）. At the sixteenth day the level of the SOD and GSH-Px had an increase （P 〈0. 01 ）, which showed its anti-oxygenation. Conclusion： PC indicated the obvious inhibition in the development of the rat cataract. The treatment period was recommended at least for fifteen days.

关 键 词：白内障 原花青素类 超氧化物歧化酶 丙二醛 谷胱甘肽过氧化物酶 

分 类 号：R285[医药卫生—中药学]