HPLC测定决明子中红镰霉素龙胆二糖苷的含量  

Determination of rubrofusarin gentiobioside in Cassia obtusifolia by HPLC

作　　者：唐力英[1] 王祝举[1] 邬秋萍[2] 赫炎[3] 黄璐琦[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]江西中医学院,江西南昌330006 [3]中国中医科学院中医临床基础研究所,北京100700

出　　处：《中国中药杂志》2008年第4期366-368,共3页China Journal of Chinese Materia Medica

摘　　要：目的：建立决明子中红镰霉素龙胆二糖苷的含量测定方法。方法：采用HPLC，Diamonsil C8柱（4.6mm×250mm，5μm），流动相乙腈-四氢呋喃-1％冰醋酸（18：3：79），流速1mL·min^-1，柱温30℃，检测波长278nm。结果：红镰霉素龙胆二糖苷在0.1-0.5μg线性关系良好（r=0.9999），平均回收率101.1％（n=5），RSD 2.2％。结论：本法快速、灵敏、准确、可靠、重复性好，可用于中药决明子药材的质量控制。Objective： To establish a method for determining the content of rubrofusarin gentiobioside in Cassia obtasifolia. Method： The HPLC with Diamonsil C18 （4.6mm×250mm,5μm） column was used, acetonitrile-THF-1% acetic acid （ 18：3：79 ） was used as a mobile phase, with flow rate of 1mL·min^-1, column temperature at 40℃ and detection wavelength at 278 nm. Result： A good linearity was obtained from 0. 1-0.5μg with r =0.9999 for rubrofusarin gentiobioside. The average recovery was 101.1%, and RSD was 2.23% （n =5）. Conclusion： The method was proved to be simple, rapid, sensitive, precise, reliable and repeatable. It can be applied to the quality control of Semen Cassia.

关 键 词：决明子 红镰霉素龙胆二糖苷 HPLC 含量测定 

分 类 号：R284.1[医药卫生—中药学]