体外联合抗原修饰的树突状细胞的抗癌效应  

Anti-tumor activity of dendritic cells modified by in vitro joint antigen

作　　者：贺子彪[1] 杨伟明[1] 焦保庭[1] 宋辉[1] 

机构地区：[1]遵义医学院附属医院普外科,贵州省遵义市563003

出　　处：《世界华人消化杂志》2008年第1期105-108,共4页World Chinese Journal of Digestology

基　　金：贵州省科技基金;No.20033040~~

摘　　要：目的:研究结肠癌患者外周血树突状细胞(dendritic cell,DC)体外联合抗原诱导后的免疫活性.方法:从结肠癌患者外周血中分离并培养DC,时效实验中分时段以肿瘤抗原修饰DC,取最佳组与T淋巴细胞共孵不同时间,用MTT检验其对肿瘤细胞的杀伤率;量效实验中将肿瘤抗原和不同浓度金黄色葡萄球菌肠毒素B(staph-lococcus aureus enterotoxin B,SEB)在上一步得出的最佳时间内修饰DC,取最佳组按不同比例(DC:T淋巴细胞)来共孵上一步得出的最佳共孵时间,用MTT检验其对肿瘤细胞的杀伤率.结果:时效实验组抗原修饰36 h组DC特有分子表达最高,对结肠腺癌细胞的杀伤率也最高,与其他组比较有差异(35.92±0.71 vs 14.85±1.24.35.92±0.71 vs 9.68±1.25.35.92±0.71 vs 17.97±1.01.35.92±0.71 vs 20.32±0.92,P<0.05),量效实验组联合抗原修饰第7天(SEB:100μg/L)DC表达DC特有分子最高,在联合修饰组内,以1:100组的杀伤率最高,与其他组比较有显著差异(47.70±2.84 vs 28.99±6.95.47.70±2.84 vs 40.02±3.65.47.70±2.84 vs 34.55±3.21,P<0.01).结论:体外DC抗原修饰和递呈的最佳时间都是36 h,联合应用超抗原SEB的最佳值100 ug/ L.DC与T淋巴细胞的最佳共孵比例是1:100.AIM： To study the immune activity of dendritic cells （DCs） in colon cancer after modification by in vitro joint antigen. METHODS： DCs were isolated and purified from peripheral blood mononuclear cells of patients with colon cancer, by combination of granulocyte/macrophage colony stimulating factor and interleukin-4. In time-efficiency experiments, DCs were modified by tumor antigen and analyzed by flow cytometry. DCs of the best group and T lymphocytes were incubated for different periods of time. The results were determined by MTT test, and the T lymphocytes were incubated for different time with the DC-destructed tumor cells. In dose-efficiency experiments, DCs were modified by tumor antigen and different concentrations of staph-lococcus aureus enterotoxin B （SEB） for the best time gained from the above step, and analyzed by flow cytometry. DCs of the best group and T lymphocytes were incubated in different proportions for the best time. The T lymphocytes were incubated with the DCs and different proportions and destructed tumor cells. RESULTS： In time-efficiency first step experiments, DCs modified by the antigen expressed especial molecules of DCs more, DCs in the 36 h group expressed them most. In time-efficiency second step experiments, the cell-killing rate was the highest in the 36 h group, and differed significantly from the other groups （35.92±0.71 vs 14.85±1.24, 35.92±0.71 vs 9.68±1.25, 35.92±0.71 vs 17.97±1.01, 35.92±0.71 vs 20.32±0.92, P 〈 0.05）. In dose-efficiency first step experiments, DCs modified by the joint antigen expressed special molecules of DCs more, DCs in the joint antigen （SEB： 100 μg/L） group expressed them the most on the seventh day; the T lymphocytes incubated with the DCs modified with joint antigen destructed more tumor cells than those incubated with the DCs modified with tumor antigen in the same proportion （P 〈 0.05）. The T lymphocytes incubated with the DCs modified with joint antigen in the proportion of 1 ： 100 destructed the m

关 键 词：树突状细胞 T淋巴细胞 金黄色葡萄球菌肠毒素B 结肠癌 

分 类 号：R446.6[医药卫生—诊断学] R512.6[医药卫生—临床医学]