黄芪总苷对小鼠日本血吸虫病肝纤维化的影响  

Effect of astrogalosides on liver fibrosis in mice with schistosomiasis japonica

作　　者：丁向东[1] 王红群[1] 吴强[1] 汪学龙[2] 黄焰[1] 张晴[1] 杨枫[1] 

机构地区：[1]安徽医科大学病理学教研室,安徽省合肥市230032 [2]安徽医科大学寄生虫学教研室,安徽省合肥市230032

出　　处：《世界华人消化杂志》2008年第2期125-131,共7页World Chinese Journal of Digestology

基　　金：安徽省高校自然科学基金重点资助项目;No.2006KJ092A~~

摘　　要：目的:研究黄芪总苷(AST)对小鼠日本血吸虫病肝纤维化和虫卵肉芽肿的影响.方法:以日本血吸虫尾蚴感染ICR小鼠制作肝纤维化动物模型,5wk末随机分为:AST高剂量组20mg/(kg·d)、AST低剂量组10mg/ (kg·d)、阳性药护肝片组540mg/(kg·d)和模型对照组,各组均于感染40d后,吡喹酮杀虫2d 500mg/(kg·d).同时设置正常对照组30只.感染后6、10和14wk每组随机处死10只,观察肝脏指数,应用HE和天狼猩红染色观察小鼠虫卵结节大小及纤维化程度;构建组织芯片,应用免疫组织化学方法检测虫卵结节中Ⅰ、Ⅲ型胶原蛋白的表达.结果:感染后10、14wk,AST高、低剂量组与模型组相比较,肝组织中血吸虫虫卵结节显著缩小,纤维化程度明显减轻,Ⅰ、Ⅲ型胶原蛋白含量(MOD)明显减低(10wk:0.093±0.002、0.084±0.003 vs 0.134±0.004.P<0.01;0.074±0.002、0.104±0.005 vs 0.146±0.008.P<0.05;14wk:0.099±0.004、0.095±0.004 vs 0.141±0.007,P<0.01:0.070±0.003、0.077±0.003 vs 0.101±0.004,P<0.05),感染10wk,AST高、低剂量组Ⅲ型胶原蛋白含量有统计学差异(P<0.01).结论:AST通过抑制虫卵结节、减少Ⅰ、Ⅲ型胶原的合成发挥抗小鼠日本血吸虫病肝纤维化的作用.AIM： To investigate the effect of astrogalosides on periovular granuloma and liver fibrosis in mice with schistosomiasis japonica. METHODS： One hundred and twenty ICR mice were infected with cercarie of Schistosoma japonicum. The infected mice were divided randomly into four groups at the end of the 5th week after infection： model group, high dose astrogaloside group （20 mg/kg per day）, low dose astrogaloside group （10 mg/kg per day） and liver protection tablet （Hugan pian） group （540 mg/kg per day） as positive drug control group. Forty days after infection, the four groups were treated with praziquantel （500 mg/kg per day） for 2 days. Thirty healthy ICR mice were used in this experiment as normal control group. Ten mice in each group were sacrificed at the end of the 6th, 10th and 14th weeks, respectively. Meanwhile, the liver index, hematoxylin and eosin stain and Sirius red stain were used to evaluate the mean area of periovular nodules and the degree of liver fibrosis. Types Ⅰ and Ⅲ collagen on tissue microarray sections were detected by immunohistochemistry. RESULTS： At the end of either the 10th or the 14th week in groups receiving two different doses of astrogalosides, the mean area of periovular nodules was decreased significantly, the degree of liver fibrosis and the expression of types Ⅰ and Ⅲ collagen （P 〈 0. 01 or P 〈 0. 05） was also reduced in comparison with those in the model group. The mean optical density values at the end of the 10th week in the high and low dose astrogaloside groups and the model group were 0.093 ± 0.002, 0.084 ± 0.003 and 0.134 ± 0.004 for type Ⅰ collagen, and 0.074 ± 0.002, 0.104 ± 0.005 and 0.146 ± 0.008 for type Ⅲ collagen, and those at the end of the 14th week in the three groups were 0.099 ± 0.004, 0.095 ± 0.004 and 0.141 ± 0.007 for type Ⅰ collagen, and 0.070 ± 0.003, 0.077 ± 0.003 and 0.101 ± 0.004 for type Ⅲ collagen. Meanwhile, there was a significant difference in the protein expression of type Ⅲ c

关 键 词：黄芪总苷 日本血吸虫病 肝纤维化 免疫组织化学 

分 类 号：R285[医药卫生—中药学]