参附注射液对大鼠肝缺血再灌注NF-κB和PGI_2/TXA_2的影响  

Effect of ginseng and prepared aconite injection on TXA_2/PGI_2 and nuclear factor-κB in rat model of hepatic ischemiareperfusion

作　　者：彭松林[1] 黄勇 顾玺[1] 赵阳[1] 戴朝六[1] 

机构地区：[1]中国医科大学附属盛京医院肝胆乳腺外科,辽宁省沈阳市110004 [2]山东省枣庄市市立医院普外科,山东省枣庄市277100

出　　处：《世界华人消化杂志》2008年第2期203-207,共5页World Chinese Journal of Digestology

基　　金：辽宁省教育厅重大应用基础研究基金;No.2004C052~~

摘　　要：目的:探讨参附注射液对大鼠肝缺血再灌注损伤的保护作用及其机制.方法:24只Wistar大鼠随机分为IR组和SF组.SF组腹腔注射参附注射液,10mL/kg.IR组大鼠给予相同剂量的生理盐水.两组均采用Pringle′s法阻断肝门缺血15min再灌注1 h、3h,测定血浆血栓素B_2(thromboxane B_2,TXB_2)、6-酮-前列腺素F1α(6-keto-PGF_(1α))、肝组织NF-κB p65表达和肝组织匀浆GSH含量,及观察肝组织形态学改变.结果:再灌注3h SF组血浆TXB_2低于瓜组(118.7±19.1 vs 386.3±282.7,P>0.05),6-keto-PGF_(1α)高于IR组(1081.7±282.7 vs 960.0±209.9,P>0.05),二者比值TXB_2/6-keto-PGF_(1α)低于IR组(0.11±0.03 vs 0.39±0.24,P<0.05):再灌注1h SF组NF-κB p65表达低于IR组(59.33%±11.06% vs 75.83%±11.46%,P<0.05);而GSH稍高于IR组(47.59±19.07 vs 37.32±4.71,P>0.05).SF组肝实质细胞和线粒体损伤明显减轻.结论:参附注射液对肝缺血再灌注损伤有保护作用,其机制与降低TXA_2/PGI_2比值,抑制NF-κB活化有关.AIM： To explore the protective effects of ginseng and prepared aconite （Shen Fu） injection on the TXA2/PGI2 and nucleus factorκB （NF-κB） in a rat model of hepatic ischemia-reperfusion （IR）. METHODS： Twenty-four male Wistar rats weighing 200-250 g were randomly divided into Shen Fu （SF） group treated with intraperitoneal SF injection （10 mL/kg） and IR group treated with 0.9% sodium chloride solution （10 mL/kg） as control group. Hepatic ischemia was induced by Pringle＇s maneuver for 15 minutes, and then reperfusion was performed for one or three hours. Venous blood samples were collected three hours after reperfusion for measurement of TXB2 and 6-keto-PGF1α. Liver tissue samples were collected one or three hours after reperfusion, for measurement of deoxidized glutathione （GSH） and morphological and immunochemical studies. RESULTS： Plasma TXB2 was lower in the SF group than in the IR group after three-hour reperfusion （118.7 ±19.1 vs 386.3 ± 282.7, P 〉 0.05）, while 6-keto-PGF1α was higher in the SF group than in the IR group （1081.7 ± 282.7 vs 960.0 ± 209.9, P 〉 0.05）. The ratio of TXB2 and 6-keto- PGF1α （0.11 ± 0.03 vs 0.39 ± 0.24, P 〈 0.05） was significantly lower in the SF group. Fifteen minutes after ischemia and one hour after reperfusion, NF-κB p65 was expressed in hepatocytes and Kuffer cells. The percentage of NF-κB p65 positive cells in the SF group was significantly lower （59.33% ± 11.06% vs 75.83% ± 11.46%, P 〈 0.05） and GSH was slightly higher （47.59 ± 19.07 vs 37.32 ±4.71, P 〉 0.05） than those in the IR group. Three-hour reperfusion fifteen minutes after ischemia caused important histologic alterations in the liver. Marked structural abnormalities were observed in the IR group, such as massive hepatocyte swelling, necrosis, mitochondria edema and vacuolar changes. In the SF group, hepatic tissue injury was significantly improved. CONCLUSION： Shen Fu injection protects hepatic tissue from ischemia-reperfusion injur

关 键 词：缺血再灌注 前列环素 血栓素A2 核转录因子ΚB 

分 类 号：R285[医药卫生—中药学]