11,12-环氧二十碳三烯酸对缺血前和再灌注前大鼠心肌钙调节蛋白的影响  

Effects of 11,12-Epoxyeicosatrienoic Acid Preconditioning and Postconditioning on Ca^(2+)-handling Proteins in Myocardial Ischemia/Reperfusion Injury in Rats

作　　者：王艳霞[1] 曾翔俊[1] 芦玲巧[1] 马立权[1] 蒋东桥[1] 穆晶[1] 王晓燕[1] 张立克[1] 唐朝枢[1] 郝刚[1] 

机构地区：[1]首都医科大学病理生理学教研室,北京100069

出　　处：《中国医学科学院学报》2007年第6期787-791,共5页Acta Academiae Medicinae Sinicae

基　　金：北京市教委科学基金(KM200710025004;KM200510025009);国家重点基础研究发展计划项目(973项目)(2006CB503807)~~

摘　　要：目的观察11,12-环氧二十碳三烯酸(11,12-EET)预处理与后处理对缺血/再灌注(IR)大鼠心肌钙调节蛋白的影响,探讨11,12-EET心肌保护的作用及其机制。方法采用Langendorff离体灌流装置,通过停灌40min/复灌30min复制大鼠心肌IR损伤模型。将33只雄性Sprague-Dawley大鼠随机分为对照组、IR组、EET预处理组(Pre-EET)及EET后处理组(Post-EET)。采用BL-420生物信号采集系统监测心功能,比色法检测肌浆网Ca2+-ATPase变化,半定量RT-PCR方法检测钙泵(SERCA)、磷酸受纳蛋白(PLB)、兰尼碱受体2型(RyR2)及1,4,5-三磷酸肌醇受体2型(IP3R2)表达变化。结果Pre-EET及Post-EET组与IR组相比,心功能改善、Ca2+-ATPase活性增高(P<0.05,P<0.01);IP3R2表达增强,PLB表达降低(P<0.05,P<0.01);Pre-EET组SERCA表达增强(P<0.05)。Pre-EET与Post-EET组间差异无显著性;各组RyR2表达差异无显著性。结论11,12-EET预处理与后处理具有拮抗IR损伤的作用,这与其诱导肌浆网IP3R2表达上调,PLB表达下调以及改善Ca2+-ATPase的活性有关;同时,预处理肌浆网SERCA表达上调,也可能是其抗IR损伤机制之一。ABSTRACT： Objective To investigate the effects of 11, 12-epoxyeicosatrienoic acid （11, 12-EET） preconditioning and postconditioning on Ca^2＋- handling proteins in myocardial ischemia/reperfusion （IR） injury in rats and reveal the effects and mechanism of 11, 12-EET on cardioprotection. Methods The IR injury model was built by stopping perfusion for 40 minutes followed by reperfusion for 30 minutes. The isolated Langendorff-perfused rat hearts were divided into 4 groups： control group, IR group, EET preconditioning （ Pre- EET） group and EET postconditioning （Post-EET） group. The computer-based electrophysiological recorder system was used to measure the changes of the maximal rate of pressure increased in the contraction phase （ ＋ dp/dtmax）, the maximal rate of pressure decreased in the diastole phase （ -dp/dtmax ）, the left ventricular end diastolic pressure （LVEDP） and the difference of left ventricular pressure （ A LVP）. The activity of Ca^2＋ -AT- Pase in sarcoplasmic reticulum was measured with colorimetric method. Reverse transcription-polymerase chain reaction was used to assess the gene expression of Ca^2＋ -handling protein [ sarcoplasic reticulum Ca^2＋ -ATPase （ SERCA）, phospholamban （ PLB）, ryanodine receptor type 2 （ RyR2 ）, and 1, 4, 5-trisphosphate inositol receptor type 2 （ IP3 R2 ） ] mRNAs level. Results Compared with IR group, the myocardial functions, the value of Ca^2＋ -ATPase, and the expressions of IP3 R2 mRNA were significantly increased and the expression of PLB mRNA was significantly decreased in both Pre-EET group and Post-EET group （ P 〈 0.05, P 〈 0.01 ）. And the expression of SERCA mRNA was significantly increased in Pre-EET group （ P 〈 0.05 ）. However, no significant differences were detected between Pre-EET and Post-EET groups. Moreover, the expression of RyR2 mRNA was not significantly different among all groups. Conclusions 11, 12-EET preconditioning and postconditioning can protect myocardium from IR injury

关 键 词：11 12-环氧二十碳三烯酸 缺血/再灌注损伤 钙调节蛋白 预处理 后处理 

分 类 号：R541.4[医药卫生—心血管疾病]