渗透胁迫下大豆幼苗根尖线粒体ATPase活性与结合态多胺含量的关系  

Relationship between ATPase Activity and Conjugated Polyamines in Mitochondria Membrane from Soybean Seedling Roots under Osmotic Stress

作　　者：胡炳义[1] 周琳[1] 杜红阳[1] 刘友良[2] 刘怀攀[1] 

机构地区：[1]周口师范学院植物学重点实验室,河南周口466000 [2]南京农业大学生命科学学院,江苏南京210095

出　　处：《种子》2008年第1期40-42,共3页Seed

基　　金：国家自然科学基金(30400280);河南省自然科学基金(0411031500)资助

摘　　要：以抗旱性不同的大豆(Glycine max L.)品种豫豆24(抗旱性较强)和豫豆6号(抗旱性较弱)幼苗为材料,研究在聚乙二醇(PEG)-6000渗透胁迫条件下,幼苗根线粒体ATPase水解活性与膜上非共价结合态多胺含量的关系。结果如下:渗透胁迫条件下,抗性强的豫豆24的ATPase活性下降的幅度明显小于抗性弱的品种豫豆6号,而豫豆24的线粒体膜上非共价结合亚精胺(Spd)的上升幅度明显大于豫豆6号。外源Spd处理,明显促进了豫豆6号在胁迫条件下非共价结合Spd含量的增加,同时抑制了胁迫条件下ATPase活性的降低,从而提高了豫豆6号幼苗的抗性;Spd生物合成的专一性抑制剂——甲基乙二醛-双(鸟嘌呤腙)(MGBG)处理,显著抑制了豫豆24在胁迫条件下膜上非共价结合Spd的增加,同时促进其在胁迫条件下ATPase活性的降低,从而明显降低了豫豆24幼苗的抗性。结果表明,线粒体膜上非共价结合的Spd可能通过维持ATPase活性的稳定来提高大豆幼苗的抗渗透胁迫能力。The relationship between ATPase activity investigated in mitochondria membrane from and contents of noncovalently conjugated-polyamines, were seedling roots of two soybean （ Glycine max L. ） cultivars, Yudou 24 （drought-tolerant） and Yudou 6 （drought-sensitive） under osmotic stress. On the 7th day,after polyethy-lene glycol （PEG） -6,000 treatment, the ATPase activity decreased more significantly in Yudou 6 than in Yu-dou 24, while the content of noncovalently conjugated-spermidine （Spd） increased more significantly in Yudou 24 than in Yudou 6. Under osmotic stress, exogenous Spd treatment resulted in enhancing the increase in non-covalently conjugated-Spd level and inhibiting the decrease in ATPase activity in Yudou 6, and enhancing its tolerance to stress. Treatment with ethylglyoxal-bis （guanylhydrazone） （MGBG）, which is an inhibitor of Spd biosynthesis,resulted in inhibiting the increase in noncovalently conjugated-Spd level and enhancing the decrease in ATPase activity in Yudou 24, and decreasing its tolerance to stress. The results suggested that the noncovalently conjugated-Spd enhance the adaptation of soybean ATPase activity in mitochondria membrane. seedlings to osmotic stress via maintenance of ATPase activity in mitochondria membrane.

关 键 词：结合态多胺 大豆 渗透胁迫 线粒体膜 ATPase 

分 类 号：S565.1[农业科学—作物学]