牡蛎软体中抗菌蛋白对白色念珠菌超微结构的影响  

Ultrastructural observation of the effect of oyster antibiosis protein on Candida albicans

作　　者：兰晓明[1] 牟茜 王春波[1] 

机构地区：[1]青岛大学医学院,山东青岛266021 [2]青岛卫生学校,山东青岛266071

出　　处：《中国海洋药物》2008年第1期55-57,共3页Chinese Journal of Marine Drugs

基　　金：863计划(2003AA625070)资助项目;山东省科技厅资助项目(22130107)

摘　　要：目的观察牡蛎软体中提取的抗菌蛋白对分离的致病性念珠菌超微结构的影响。方法取临床分离的白色念珠菌,暴露于抗菌蛋白中24h,应用透射电镜观察细胞超微结构的变化。结果经4h抗菌蛋白(3.5×103U.mL-1)作用,白色念珠菌菌体变为椭圆形,细胞壁松弛,细胞质固缩;16h后,菌体呈圆球形,细胞壁边缘呈毛状,残缺并且明显肿胀,胞浆内容变空,细胞器几乎消失,胞核不清;24h后,菌体呈不规则形,细胞壁缺失,细胞质解体出现空腔。结论抗菌蛋白对白色念珠菌有较强的抗菌作用。Objective To observe the ultrastructural changes of Candida albicans by the effect of oyster antibiosis protein which was extracted from muscle of oyster. Methods The colonies of candida aIbica,zs exposed to oyster antibiosis protein for 24 hours, with a blank control group and the differences of their ultrastrueture were studied under electron microscope. Results After affected by oyster antibiosis protein for 4h, the cell walls were lacked and had reduetus with pyknotic cytoplasm. 16 hours later, most cells became global shape and their cvtomatrix components were lost and some vacuoles appeared in the cytoplasm. 24 hours later, the cell walls depleted and the cells spitted. Conclusion oyster antibiosis protein affected commendably the function of candida albicans.

关 键 词：牡蛎 抗菌蛋白 念珠菌 超微结构 

分 类 号：R931.74[医药卫生—生药学] R965[医药卫生—药学]