昆布多糖对大鼠减肥及降血脂作用的实验研究  

Experimental Study on Weight-losing and Hypolipidemic Effects of Thallus laminariae Polysaccharides and Its Mechanisms in Rat

作　　者：王慧铭[1] 孙炜 黄素霞[3] 项伟岚[3] 

机构地区：[1]浙江中医药大学药学院,杭州310053 [2]浙江省中医药学会,杭州310003 [3]浙江大学医学院,杭州310031

出　　处：《中国现代应用药学》2008年第1期16-19,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省中医药管理局2002年科研项目(2002C060)

摘　　要：目的研究昆布多糖(Thallus laminariae polysaccharides,TLP)对大鼠减肥、降血脂的作用及其机制。方法制作肥胖大鼠模型,随机分成六组,分别以蒸馏水、TLP(100,200,300mg·kg-1·d-1)、奥利司他(orlistat,赛尼可,60mg·kg-1·d-1)和洛伐他汀(lovastatin,10mg·kg-1·d-1)灌胃,为期40d,在实验过程中,按时检测相关指标。结果TLP能明显降低肥胖大鼠的体重、减少大鼠腹腔、肾、生殖器周围脂肪、降低Lee’s指数和肝、肾重量(P<0.05),而且其作用与减肥药赛尼可在统计学上无显著性差异(P>0.05)。TLP能明显降低肥胖大鼠血清三酰甘油(TG)、胆固醇(TC)、提高高密度脂蛋白胆固醇(HDL-C)水平(P<0.05),而在实验期内低密度脂蛋白胆固醇(LDL-C)变化不明显(P>0.05),而且其作用与降脂药洛伐他汀比较无显著性差异(P>0.05)。TLP能明显改善肥胖大鼠血清卵磷脂胆固醇脂酰基转移酶(LCAT)、脂蛋白脂酶(LPL)和胰脂肪酶(PL)活性(P<0.05),而对肝脂肪酶(HL)活性在实验期间影响不明显(P>0.05)。结论TLP对肥胖大鼠具有明显的减肥作用,该作用与常用的减肥药赛尼可比较无显著性差异。TLP同时能降低血清甘油三酯和总胆固醇、改善血清HDL-C水平,而且该作用与降脂药洛伐他汀比较无显著差异。其作用机制可能与增强LCAT、LPL、PL酶活性有关。OBJECTIVE To study the functions of Thallus laminariae polysaccharides （TLP） and its mechanisms on experimental fatty rats, METHODS Male SD strains rats （50 - 70 g） were randomly divided into the normal group（A） fed normal diet（ 10 rats） and model groups（60 rats） fed high-lipids-diet. After eight weeks, the models were prepared successfully, and they were redivided into six groups ： the control group （ Ⅰ ）, the low-dosage group （ Ⅱ ）, the medium-dosage group （ Ⅲ ）, the high-dosage group （ Ⅳ ） , the Orlistat group（ Ⅴ ） and the lovastatin group（Ⅳ） （ 10 rats each）, Group I was fed with water while Group Ⅱ , Ⅲ, Ⅳ were administrated with TLP at the dosages of 100 mg·kg^-1 ,200 mg·kg^-1 and 300 mg·kg^-1 , Group Ⅴ was treated with the drug of Orllstat at the dosage of 60 mg·kg^-1 and Group VI was treated with the drug of lovastatin at the dosages of 10 mg·kg^-1. The experiment lasted 40 days. During the course, the body weight was tested every 10 days, and the fatty tissue, the weight of liver ＆ kidney and the Lee＇s Index,the fast blood TG, TC, low-density cholesterol （LDL-C）, high-density cholesterol （HDL-C）, were tested at the 40^th day. In addition, at the 40^th day the activity of the fast blood LCAT, LPL, PL＆HL were also determined. RESULTS The body weight ,the fatty tissue, the weight of liver ＆ kidney and the Lee＇ s Index ,including TG, TC level of Group Ⅱ,Ⅲ,Ⅳ was decreased significantly compared with that of before administration and Group Ⅰ （ P 〈 0, 05 ）. However, there was no difference in Group Ⅱ , Ⅲ, Ⅳ （ P 〉 0.05 ）. TLP played an important role in increasing the concentration of blood HDL-C （P 〈 0.05 ）. However, there was on significant effect on the concentration of the fast blood LDL-C, TLP could improved the activities of LCAT, PL and LPL significantly （ P 〈 0.05 ）. No sig- nificant differences were observed in Group Ⅱ , Ⅲ, Ⅳ（ P 〉 0.05 ）, However the activity of HL

关 键 词：昆布多糖 肥胖 高脂血症 胆固醇 大鼠 

分 类 号：R917.791[医药卫生—药物分析学] R972.6[医药卫生—药学]