液相色谱串联质谱法测定人血浆中硝苯地平的浓度  

Quantitation of Nifedipine in Human Plasma by LC-MS/MS

作　　者：刘拥军[1] 王秀丽[1] 栾杨[1] 姚庆强[1] 

机构地区：[1]山东省医学科学院药物研究所,济南250062

出　　处：《中国现代应用药学》2008年第1期51-53,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立测定人血浆中硝苯地平浓度的LC—MS／MS方法。方法血浆样品经液液提取后，以甲醇（含0．1％乙酸）：水（含0．1％乙酸＋2．5mmol·L^-1甲酸胺）：80：20为流动相，使用Agilent1100VL型离子阱质谱仪，电喷雾离子源正离子模式，采用多反应离子监测方式测定硝苯地平（MRM，m／z347→315）。结果血浆中硝苯地平的线性范围为1．0～100．0ng·mL^-1。定量下限为1.0ng·mL^-1。准确度在85％～115％之间，日内、日间精密度（RSD）在±15％之内。结论该方法准确，特异性强，可用于硝苯地平药动学研究。OBJECTIVE To establish a LC-MS/MS method to determine nifedipine in human plasma. METHODS After a L/L extraction,the plasma samples were separated on a Zorbax SB-C18 （ 150 mm × 4.6 mm） column with methanol（ content 0. 1% HAc）- H2O （ content 0.1% HAc ＋ 2.5 mmol · L ^-1 ammonium formate） （ 80：20 ） as mobile phase. Electrospray ionization source （ESI） was applied and operated in positive ion mode. Multiple reactions monitoring （MRM） mode was used and nifedipine was quantified by monitoring the ion transition of m/z 347→315. RESULTS The linear calibration curves were obtained over the concentration range of 1.0 - 100 ng · mL^-1 in plasma. The lower limit of quantitation was 1,0 ng · mL^-1. Accuracy was within 85% - 115% ,within day and between day precision were within ± 15%. CONCLUSION The method proved to be accurate and specific,and can be applied to pharmacokinetic study of nifedipine.

关 键 词：硝苯地平 液相色谱串联质谱 药动学 

分 类 号：R972.4[医药卫生—药品]