电针对大鼠创伤痛术后抗炎/促炎细胞因子比值影响的昼夜节律研究  

Circadian Rhythm of Electro-acupuncture Efficacy in Ratio of Antiinflammatory and Proinflammatory Cytokine of Rats Suffering from Traumatic Pain

作　　者：薛红[1] 徐蕾[1] 余曙光[1] 宋开源[1] 唐勇[1] 吴巧凤[1] 魏焦禄[1] 郭玲玲[1] 吴林锦[1] 

机构地区：[1]成都中医药大学,四川成都610075

出　　处：《成都中医药大学学报》2007年第4期22-27,共6页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：四川省教育厅重点科研项目(编号:2006A047)

摘　　要：目的:研究创伤痛应激所致的免疫抑制及其昼夜节律变化,以及针刺抗创伤痛所致免疫抑制的效应是否也具有昼夜节律。方法:选择经过痛阈筛选择的192大鼠,将动物随机分为24组,每组8只,其中每4组作为1个观察时间点,每组8只动物,分别于6个相应时间点对动物进行处理:空白对照组(不截肢,不电针)、空白针刺组(不截肢,仅电针)、截肢模型组(只截肢,不电针)、截肢电针组(截肢,同时处以电针足三里、环跳穴),处理结束后24 h在相应时间点处死动物,采集样本,用放射免疫法测电针对截肢大鼠血浆抗炎/促炎细胞因子(IL-10/IL-6)比值的昼夜节律的影响。实验时同一时间点的4个组的手术、电针及动物处死、取样均同时进行。结果:针刺能提高正常大鼠IL-10/IL-6比值,截肢后比值明显降低,截肢时电针能升高IL-10/IL-6比值,但仍较正常组低。从节律方面看,空白对照组血浆IL-10浓度具有一定的昼夜变化趋势,电针后IL-10浓度有所下降,仍具有明显昼夜波动;截肢后血浆IL-10浓度明显下降,昼夜节律不明显;截肢电针组血浆IL-10浓度较空白对照组、空白针刺组有所下降,但较截肢模型组高。空白对照组血浆IL-6浓度呈现双峰结构的变化趋势,电针后IL-6浓度有所上升,变化趋势不规律,表现为波动不大,未见明显的昼夜节律变化;截肢后血浆IL-6浓度明显升高,出现明显昼夜节律,其最大值出现在下午16时左右,最小值值出现在凌晨4点左右;截肢时电针,动物血浆IL-6浓度较截肢模型有轻度下降。结论:电针对血浆IL-10/IL-6具有双向调节作用。针刺作为一种较轻微的应激刺激,对正常动物的免疫系统功能有一定影响,且能对抗创伤痛所致的免疫抑制,且这种抗免疫抑制效应具有一定的昼夜节律。Abstract： Objective： To understand the circadian rhythm of electro - acupuncture efficacy in the treatment of immune suppression, the variability of electro - acupuncture efficacy at different time points in a 24 - hour period. Methods： Rats （192） with pain threshold exam bolting were, without prejudice, divided into 24 groups with 8 in each group. For the subsequent experiments, four groups were used for each time point, and six different time points were then chosen as8：00 （ZTO）, 12：00 （ZT4）, 16：00 （ZT8）, 20：00 （ZT12）, 24：00 （ZT16） and4：00 （ZT20）. Rats were then subject to different treatments, （1）, no amputation （AMP） and electro -acupuncture （Control group） ; （2）, no AMP, but with electro -acupuncture on points ST36 and GB30 （Electro -acupuncture Group）; （3）, AMP but no electro- acupuncture （AMP Group）; AMP and electro- acupuncture treatment on points ST36 and GB30 （AMP -Electro -acupuncture Group）. At each time point, tail -flick method was employed to evaluate the pain threshold. The plasma method. Results： The ratio of plasma anti levels of IL - 10 and IL - 6 were determined by using radio - immunity - inflammatory to pro - irfflamatory cytokine levels （ IL - 10/IL - 6 ） in rats of control group has a obvious circadian rhythm. As immune suppression results from traumatic pain, electro - acupuncture has lesser effects on the rats＇ immune system in Electro - acupuncture Group and therefore has greater various regulatory effects at different time points. When compared with the AMP group, most of rats in AMP - electro - acupuncture group showed increasing IL - 10 levels at 8 am and decreasing IL - 6 levels on 8pm. The most marked effects of electro - acupuncture on the ratios of IL - 10/IL - 6 were observed at 8am and 8pro. Further, electro- acupuncture could raise the thymus gland exponent of insulted rat to different extent at different time points showing the biggest increase at 12am and 8pm. Conclusion： The electro - a

关 键 词：择时治疗 针刺镇痛 昼夜节律 免疫抑制 IL-6 IL-10 放射免疫法 

分 类 号：R245.3[医药卫生—针灸推拿学] R817.9[医药卫生—中医临床基础]