光线性角化病皮损的细胞DNA含量流式细胞分析  

Analysis of Cellular DNA Content of Skin Lesions in Actinic Keratosis with Flow Cytometry

作　　者：李玉平[1] 廖海鹰[2] 孙修蓉[1] 王凤荣[3] 四荣联[1] 姚春华[1] 张贵贞[1] 张玉敏[4] 

机构地区：[1]河北医科大学第二医院皮肤科,050000 [2]河北医科大学第二医院外科,050000 [3]河北医科大学基础所病理室 [4]河北医科大学第二医院病理科,050000

出　　处：《中华皮肤科杂志》1997年第4期246-247,共2页Chinese Journal of Dermatology

摘　　要：光线性角化病的病理组织学特征为不同程度的表皮细胞的非典型增生。为了更加客观地反映该病的这一特征,应用流式细胞技术定量分析了27例光线性角化病皮损细胞的DNA含量及细胞增殖活性。结果:光线性角化病细胞的增殖指数(PI)高于正常人皮肤的PI值(P<0.05),8例非典型增生严重的光线性角化病细胞的DNA指数(DI)高于其余19例光线性角化病的DI值(P<0.05)。提示:光线性角化病皮损细胞增殖活跃,细胞非典型增生程度越高,DNA含量异常越明显。Actinic keratosis (AK) is a precancerous state of the skin, which shows epidermal dysplasia of different degrees. The cellular DNA content and proliferative activity of the skin lesions in 27 cases of AK were analyzed with flow cytometry. The results showed that the PI (proliferating index) in AK was higher than that in normal skin (P<0.05). Among 27 eases of AK, the cellular DI (DNA index) of 8 cases of severely dysplastie AK was higher than the rest 19 cases (P<0.05). The results suggest that the cells in the skin lesions of AK proliferate actively, and the higher the degree of dysplasia showed in AK the more the significance of aberration of DNA content.

关 键 词：光线性角化病 皮损细胞 DNA 流式细胞术 

分 类 号：R758.14[医药卫生—皮肤病学与性病学]