氟美松对激素抵抗型哮喘患者外周血单个核细胞的影响  

INHIBITION OF PERIPHERAL BLOOD T CELL PROLIFERATION BY DEXAMETHASONE IN STEROID RESISTANCE ASTHMA

作　　者：姚彬[1,2,3] 李明文[1,2,3] 朱元珏 田文[1,2,3] 李如臻 许仁和 

机构地区：[1]山东省烟台市毓璜顶医院 [2]中国医学科学院中国协和医科大学北京协和医院 [3]山东医科大学附属医院

出　　处：《中华内科杂志》1997年第8期519-521,共3页Chinese Journal of Internal Medicine

基　　金：山东省卫生厅青年科研基金

摘　　要：为探讨激素抵抗（ＳＲ）型哮喘的发病机制，应用植物血凝素（ＰＨＡ）对淋巴细胞的增殖试验，观察不同浓度的氟美松对淋巴细胞增殖的抑制作用，并采用放射免疫法（ＲＩＡ）测定血浆皮质醇浓度。结果显示：氟美松对ＳＲ型哮喘患者淋巴细胞增殖的抑制作用明显低于激素敏感（ＳＳ）型哮喘患者（Ｐ＜０．０５）；ＳＲ型、ＳＳ型哮喘患者的血浆皮质醇浓度明显低于正常对照组（Ｐ＜０．０１），ＳＲ型又明显低于ＳＳ型（Ｐ＜０．０５）。提示：（１）ＳＲ型哮喘患者的淋巴细胞对糖皮质激素的抑制作用弱，反应性降低；（２）ＳＲ型哮喘患者的血浆皮质醇浓度明显降低与长期大量应用糖皮质激素有关。We investigated the pathogenesis of steroid resistance (SR). The inhibition of phytohemagglutininum induced T lymphocyte proliferation by dexamethasone (Dex) was investigated by lymphocyte proliferation assay. Plasma cortisol concentration was measured by radioimmune lectrophoresis autoradiography kit. The inhibition effects of Dex (10 -7 mol/L) on the proliferation of lymphocytes from SR asthmatics were significantly less than those from steroid sensitive (SS) asthmatics ( P <0.05). Plasma cortisol concentrations of SR asthmatics were significantly lower than those of normal subjects ( P <0.01) and SS asthmatics ( P <0.05). There was no correlation between the degree of inhibition by Dex (10 -7 mol/L of SR, SS asthmatics of proliferation of T lymphocytes and plasma cortisol concentration. T lymphocytes from SR asthmatics were insensitive to Dex. Plasma cortisol concentration from SR asthmatics were significantly lower than those of SS asthmatics, that may be due to the large dose of glucocorticoids given in long time.

关 键 词：哮喘 糖皮质激素 氟美松 药物疗法 单核细胞 

分 类 号：R562.250.5[医药卫生—呼吸系统]