吸毒人群和HIV-1感染者人群DC-SIGN／DC-SIGNR基因多态性分析  

Analysis of genetic polymorphisms in DC-SIGN and DC-SIGNR among drug users and HIV-1 carriers

作　　者：赵广录[1] 冯铁建[1] 王辉[2] 赵锦[1] 王晓辉[1] 石向东[1] 柯跃斌[1] 周伯平[2] 朱托夫 

机构地区：[1]深圳市疾病预防控制中心艾滋病防制科,广东深圳518020 [2]深圳市东湖医院,广东深圳518020 [3]Department of Laboratory Medicine, University of Washington School of Medicine, WA 98195, U. S. A

出　　处：《中国艾滋病性病》2008年第1期21-24,共4页Chinese Journal of Aids & STD

基　　金：美国华盛顿大学CFAR基金资助[编号P30AI-27757-18(S3)];深圳市2005科技计划重点项目资助(编号JH200505270384A);广东省医学科研基金(A2005633)

摘　　要：目的研究等位基因DC-SIGN/DC-SIGNR在人类免疫缺陷病毒Ⅰ型(HIV-1)感染者与HIV-1阴性吸毒人群中的分布,筛选出HIV-1抗性基因。方法选择深圳市戒毒所HIV抗体阴性戒毒者的样本257份,HIV-1感染者的样本220份。提取外周血基因组DNA,对DC-SIGN/DC-SIGNR颈区基因进行特异性聚合酶链反应(PCR)扩增,PCR扩增产物行琼脂糖凝胶电泳检测和基因序列测定分析。结果通过统计学分析发现,DC-SIGNR颈区中杂合子7/5、7/6和7/7在HIV阴性吸毒者中出现的频率为13.33%、7.50%、49.4%,在HIV-1感染者中出现的频率为11.05%、4.65%、51.74%,两者无明显差异。说明DC-SIGN在吸毒者与HIV-1感染者中的基因型及等位基因的分布无明显差异。结论深圳市吸毒人群中DC-SIGNR和DC-SIGN基因型分布和等位基因出现频率,与HIV-1感染者无显著性差异,推测DC-SIGNR和DC-SIGN基因多态性对HIV-1易感性无明显影响。Objective To study the distribution of DC-SIGN-SIGNR alleles among drug users（DUs） and HIV-1 carriers in Shenzhen,and to evaluate the role of these alleles in the construction of genetic resistance to HIV-1 and screen anti-HIV-1 genes in Shenzhen.Methods The genomic DNA from whole blood samples collected from 257 DUs in the Shenzhen Detoxification Center and 220 HIV-1 infectors identified in Shenzhen was amplified by PCR-DC-SIGN/DC-SIGNR neck domain repeat regions, and the PCR products were sequenced directly and analyzed by agarose gel elec- trophoresis. Pearson＇ s X2 test was used for statistic analysis. Results This study showed that the frequency of DC-SIGNR heterozygous 7/5,7/6 and 7/7 in DUs was 13.33%, 7.50% and 49.4%, respectively and that in HIV-1 carriers was 11.05 %, 4.65 % and 51.74 %. There was no significant difference between DUs and HIV- 1 carriers, neither significant difference was found in the DC-SIGN repeat region based on numbers of repeats between DUs and HIV-1 carriers.Conclusion There is no significant difference in DC-SIGNR and DC-SIGN genotype distribution and allele distribu- tion frequency between DUs and HIV-1 carriers.This suggests that DC-SIGNR and DC-SIGN polymorphisms display no significant influence on HIV-1 infection susceptibility.

关 键 词：人类免疫缺陷病毒Ⅰ型 基因多态性 突变 

分 类 号：R373.9[医药卫生—病原生物学]