免疫印迹法检测过敏原特异性IgE  

Detection of Allergen-specific IgE with Immunobloting

作　　者：赵俊芳[1] 王学谦[2] 丁红梅[1] 孙晓慧[3] 潘春莹[4] 

机构地区：[1]天津医科大学,天津300120 [2]天津医院,天津300000 [3]天津长征医院,天津300120 [4]天津儿童医院,天津300000

出　　处：《现代检验医学杂志》2008年第1期56-58,共3页Journal of Modern Laboratory Medicine

摘　　要：目的研制过敏原特异性IgE检测试纸条,对该试纸条的检测效能进行评估。方法将不同浓度的过敏原溶液点样到固相载体-硝酸纤维素膜上,经过固定,封闭,洗涤步骤,加入待检血清,血清中的抗体与膜上的抗原反应,经酶-底物反应显色,根据颜色判读结果,并与Allergy Screen过敏原检测系统进行对比。结果该法的灵敏度和特异性较好,与Allergy Screen检测系统符合率较高。食物过敏原检测阳性率高于Allergy Screen法。结论用硝酸纤维素膜检测过敏原特异性lgE抗体,具有样本血清量少,操作简便,价格低廉等优点,具有较强的临床检测应用价值,为体外过敏原检测提供了新的思路。Objective To develop allergen-specific IgE detection strip,and evaluate the detection performance of this strip. Methods Spotted allergen solutions of different concentration onto solid carrier-nitrocellulose filters, and serum was added after fixture ,blockage and washing. Result was determined by coloration induced by enzymatic reactivity. Results This method corresponded well with Allergy Screen detection system. Its food allergen detection positive rate was higher than that of Allergy Screen system. Conclusion The method developed has such advantages as few serum sample volume, simple procedure,low price, good specificity and sensitivity. Therefore, it has high clinical application value, and provide new thought for allergen detection in vitro.

关 键 词：过敏原特异性IGE 硝酸纤维素膜 免疫印迹 

分 类 号：R446.6[医药卫生—诊断学]