TRFIA与ELISA法检测乙肝病毒血清学标志物结果的比较  

Comparison of HBV Serum Marker Results between TRFIA and ELISA

作　　者：黄宪章 石文 庄俊华 丁海明 潘婉仪 

机构地区：[1]广州中医药大学第二附属医院二沙岛分院检验科,广州510105

出　　处：《现代检验医学杂志》2008年第1期59-61,共3页Journal of Modern Laboratory Medicine

基　　金：国家高技术研究发展计划(863计划)课题资助(2004AA2Z3762)

摘　　要：目的探讨时间分辨荧光免疫测定法(TRFIA)定量检测乙型肝炎血清学标志物(HBVM)与酶联免疫吸附试验(ELISA)定性检测HBVM的关系及其临床意义。方法采用TRFIA和ELISA法分别检测161份血清标本HBVM。结果对于高浓度的HBVM血清,两种方法检出率有较高的一致性,但是对于稍高于临界值的血清,TRFIA检出率明显高于ELISA。结论TRFIA检测HBVM比ELISA法敏感。Objective To explore the clinical value and relationship between time-resolved fluoroimmunoassay（TRFIA） to detect HBV M in quantity and enzyme linked immunosorbent assay（ELISA） to detect HBV M in quality. Methods 161 serum specimen were collected to detect HBV M by TRFIA and ELISA. Results The results of TRFIA were accorded to that of ELISA for high titre HBV M specimen. The positive results of TRFIA were higher than that of ELISA for nearly cut-off value HBV M specimen. Conclusion TRFIA is more sensitive than ELISA.

关 键 词：乙型肝炎病毒 血清学标志物 时间分辨荧光免疫测定法 酶联免疫吸附试验 

分 类 号：Q503[生物学—生物化学] R446.62[医药卫生—诊断学]