自制SDMC-1液对热缺血冷保存狗心保护的实验研究  

Preservation of Canine Hearts after Warm Ischemia (6 Minutes)and Hypothermic Storage(6 Hours)with SDMC 1 Solution for Transplantation

作　　者：毕研文[1] 宋惠民[1] 李守先[1] 徐巨林[1] 张延恩[1] 

机构地区：[1]山东医科大学附属医院心外科

出　　处：《中华胸心血管外科杂志》1997年第4期243-246,共4页Chinese Journal of Thoracic and Cardiovascular Surgery

摘　　要：为提高心脏离体保存效果，作者研制了ＳＤＭＣ１号（山东医科大学心脏１号）心脏保存液。实验采用狗心异体异位移植。供心先经６分钟常温缺血后，实验组（ｎ＝６）用ＳＤＭＣ１液，对照组（ｎ＝６）用Ｃｏｌｉｎｓ液分别进行心脏灌洗并离体低温保存（４℃）６小时。观察指标：（１）供心再供血至恢复稳定节律时间；（２）电除颤次数；（３）血清肌酸激酶同功酶（ＣＫＭＢ）和丙二醛（ＭＤＡ）；（４）供心左心室功能和心肌超微结构。结果显示：ＳＤＭＣ１液灌洗并单纯低温离体保存的狗心移植后，心电活动和左心室功能恢复快，较Ｃｏｌｉｎｓ液有更好的心肌保护效果。Aim:How to prolong the safe preservation time of the donor heart for heart transplantation remains an important problem.For this reason,a new heart preservation solution,SDMC 1 Solution (Shandong Medical University Cardiosol 1)was prepared according to our own receipe.This study was designed to compare the protective and preservation effect of SDMC 1 solution with that of Collins' solution on canine hearts.Method:In 12 dogs,the hearts were subjected to 6 minute warm ischemia and anoxemia in situ and were then perfused with cold SDMC 1 solution(n=6)and Collins' solution (n=6) respectively for myocardial protection and the hearts were immediately removed and separately preserved in the aforementioned solutions at 4℃ for 6 hours.Heterotopic allotransplantations were promptly performed with all the canine hearts preserved.Results:Significant differences were observed between two groups of transplanted canine hearts:(1)The mean time intervals between reperfusion to a stable rhythm was shorter in SDMC 1 group than Collins' group;(2)Rate of intraoperative defibrillation was higher in Collins' group;(3)The mean values of the postoperatively released creatine kinase MB (CK MB) and malondialdehyde (MDA) were greater in Collins' group;(4)More water was present in the myocardium in Collins' group;(5)Better recovery of left ventricular function and milder reperfusion injury in SDMC 1 group.Conclusion:SDMC 1 solution is better than Collins' solution in myocardial protection and heart preservation in dogs.

关 键 词：热缺血 心脏保存 心脏移植 

分 类 号：R654.2[医药卫生—外科学]