金属硫蛋白对犬心肌缺血/再灌注损伤的保护作用(ESR及心肌细胞超微结构立体定量分析)  

A Protective Effect of Metallothionein on Post ischemic Reperfused Canine Heart—ESR Spectra Monitoring and Morphometric Analysis of Myocardial Ultrastructures

作　　者：杨泽泉[1] 李曰民[1] 程时[1] 陈鸿义[1] 张明礼[1] 

机构地区：[1]北京医科大学第一医院胸外科

出　　处：《中华胸心血管外科杂志》1997年第4期247-249,共3页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：国家自然科学基金

摘　　要：实验采应用电子顺磁共振技术及细胞超微结构立体定量分析方法，研究金属硫蛋白（ＭＴ）对犬心肌缺血／再灌注损伤的作用。１０只杂种犬均分为对照组ＭＴ保护组，低温体外循环下心肌缺血１２０分钟。ＭＴ保护组的心脏停跳液中含ＭＴ５．０×１０６ｍｏｌ／Ｌ。结果显示，心肌缺血后再灌注损伤在再灌注早期即已非常明显；氧自由基是导致再灌注损伤的原因之一。ＭＴ能清除再灌注早期产生的氧自由基，减轻心肌缺血后再灌注损伤。The effect of metallothionein(MT)on post ischemic reperfused canine hearts was studied by monitoring myocardial electronic spin resonance (ESR) and the changes in myocardial ultrastructures.Ten hybrid dogs were randomized evenly into control group and MT group.In MT group,MT was added to St.Thomas Hospital Cardioplegia solution with a final concentration of 5.0×10 6 mol/L.After 120 minutes of hypothermic global ischemia,mild myocardial injury was found in both groups.At 20 seconds after aortic declamping,more oxygen derived free radicals(OFR) were detected and more severe injury to the myocardium was found in control group.But in MT group,all parametors were in the same ranges as those found at the end of ischemia.The data suggested that OFR contributed to the reperfusion injury.MT had a capacity to scavenge OFR,and thus produced a protective effect on post ischemic reperfused heart.

关 键 词：心肌缺血 再灌注损伤 金属硫蛋白 心肌保护 

分 类 号：R654.2[医药卫生—外科学] R977.6[医药卫生—临床医学]