微量细胞培养法在病毒性肺炎病原学检测中的应用  

Application of microplate method to virus isolation in viral pneumonia

作　　者：成焕吉 傅文永[1,2] 乔红梅 李晓东[1,2] 鲁继荣 梁东[1,2] 郭慧君 

机构地区：[1]吉林省儿科研究所 [2]白求恩医科大学第一临床医学院儿科

出　　处：《中华儿科杂志》1997年第8期399-401,共3页Chinese Journal of Pediatrics

摘　　要：为了探索病毒分离新技术，采用微量细胞培养法与试管法，对３２５例肺炎患儿的咽分泌物进行了病毒分离的对比研究。结果微量细胞培养法共分离出１２１株病毒，分离率３７．２％；其中腺病毒５１株，呼吸道合胞病毒３４株，流感病毒Ａ９株，副流感病毒２２株，柯萨奇病毒５株。试管法共分离到病毒１０１株，分离率为３１．０％。两种方法相比，微量细胞培养法病毒分离用时短，可同时分离出多种病毒；同时可节省大量实验材料，重复性好。因此是一种操作简便，省时省物、准确可靠的病毒分离方法，值得推广应用。To elucidate a new technique for virus isolation, the authors conducted a comparative study on 325 pharyngeal secretions using the microplate method and the tube method. Of them 121 strains of virus (positive rate 37.2%) were isolated with microplate method, including adenovirus (51 strains), respiratory syncytial virus (34 strains), influenza A virus (9 strains), parainfluenza virus (22 strains) and coxsackie virus (5 strains), while 101 strains of virus were isolated with the tube method (positive rate 31.0%). The microplate method is superior to the tube method in its efficient, reproducible, simple, convenient, time saving and reliable features and is worthy of applying in a large scale.

关 键 词：病毒性肺炎 肺炎 病原学 细胞培养法 

分 类 号：R563.1[医药卫生—呼吸系统]