人参抑制小鼠胸腺细胞凋亡的实验研究  

INHIBITORY EFFECT OF GINSENG ON APOPTOSIS IN MOUSE THYMUS BY ADMINISTRATION OF LIPOPOLYSACCHARIDE(LPS)

作　　者：牛建昭[1,2] 魏育林[1,2] 曹炜[1,2] 金恩源 

机构地区：[1]北京中医药大学组织学胚胎学教研室 [2]中日友好医院临床研究所

出　　处：《解剖学报》1997年第3期270-275,共6页Acta Anatomica Sinica

基　　金：人事部留学回国人员科技活动资助

摘　　要：用大肠杆菌脂多糖（ＬＰＳ）所致小鼠胸腺细胞凋亡模型和琼脂糖凝胶电泳、流式细胞术及免疫组织化学等方法，研究不同浓度高丽人参水提取物体内对小鼠胸腺细胞的影响。结果表明，每只小鼠腹腔注射１０～２０ｍｇ人参水提取物可防止ＬＰＳ所致小鼠体重及胸腺减重，提高胸腺细胞存活力，抑制胸腺细胞ＤＮＡ降解和促凋亡蛋白Ｂａｘ的过量表达，免疫组织化学研究结果在蛋白水平上初步解释了人参抑制小鼠胸腺细胞凋亡的作用机制。Defective regulation of apoptosis may play a important role in the aetlology of cancer, AIDS, autoimmune diseases, and degenerative diseases of the central nervous system. The pharmacological manipulaiton of apoptosis offers new possibilities for the prevention and treatment of these lllnesses. ginseng, one of the valuable traditional chinese medicine, has many pharmacologic effects. In present study, we injected (i.p.) ginseng extract (GS) to the BALB/c mice administrated with bacterial lipopolysaccharide (LPS) and observed the effects of genseng on thymus weight, cell viability and DNA fragmentation confirmed by agarose gel electrophoreses, flow cytometry (FCM) and expressioin of Bax protein by immunocytochemistry. The results shown that (1) LPS could cause the decreasion of thymus weight, thymocyte number and viability and induced thymocytic apoptosis in vivo. (2) There were no significant difference between ginseng control group and normal control group. (3) Ginseng could increase the thymus weight, thymocyte number and viability of the mice administrated with LPS.(4) DNA fragmentation and apoptotic thymocytes iin the thymus of GS+LPS group was less than that in LPS group. (5) By immunocytology and image analysis, staining of bax protein in thymocytes of GS+LPS group was weaker than that in LPS group. These results indicate that ginseng could inhibite thymocytic apoptosis of the mice administrated with LPS.

关 键 词：细胞凋亡 胸腺细胞 大肠杆菌脂多糖 人参 

分 类 号：R285.5[医药卫生—中药学] R329.28[医药卫生—中医学]