促性腺激素释放激素 GnRH 相关肽存在于培养的大鼠垂体前叶促性腺激素细胞  

GnRH ASSOCIATED PEPTIDE EXISTS IN CULTURED RAT ANTERIOR PITUITARY GONADOTROPHS

作　　者：宋天保[1] 葛玲[1] 王丽蓉[1] 姚向民[1] 

机构地区：[1]西安医科大学组织学胚胎学教研室

出　　处：《解剖学报》1997年第3期294-296,共3页Acta Anatomica Sinica

摘　　要：为了探讨垂体促性腺激素细胞中ＧｎＲＨ相关肽（ＧＡＰ）的来源，本实验选用两种特异性抗ＧＡＰ抗体和抗ＦＳＨ－β抗体，采用免疫细胞化学双重染色技术，研究了ＧＡＰ在培养的大鼠垂体前叶细胞内的分布和定位。结果发现一些培养细胞可表达ＧＡＰ，而且ＧＡＰ免疫反应产物和ＦＳＨ免疫反应产物共存于同一种细胞。这表明ＧＡＰ存在于培养的大鼠垂体前叶促性腺激素细胞内。本文讨论了ＧＡＰ的来源及其可能的生物学作用。In order to investigate the origin of GnRH associated peptide (GAP) in the pituitary gonadotrophs, the localization of GAP in the cultured cells from the rat anterior pituitary was studied by using two specific anti GAP antibodies and anti FSH β antbody and immunocytochemical double staining technique. Some cultured cells were found to express GAP, and GAP and FSH immunoreactive products were located in the same cells. The results indicated that GAP was present in the cultured rat anterior pituitary gonadotrophs. The origin and possible biological roles of GAP were discussed in this paper.

关 键 词：GnRH相关肽 促性腺激素细胞 细胞培养 垂体 

分 类 号：R322.54[医药卫生—人体解剖和组织胚胎学]