工频磁场对离体细胞胞浆钙离子浓度的影响  

Effects of 50 Hz Ferromagnetic Field on the Concentrations of Intracellular Calcium-ion in vitro

作　　者：郭鸿涌[1] 郑末[2] 邢凌霄[3] 樊长在[1] 张祥宏[3] 颜威利[1] 

机构地区：[1]河北工业大学电气与自动化学院,天津300130 [2]天津市第五中心医院病理科,天津300450 [3]河北医科大学病理研究室,河北石家庄050017

出　　处：《河北大学学报（自然科学版）》2008年第1期34-38,共5页Journal of Hebei University(Natural Science Edition)

基　　金：河北省自然科学基金资助项目(502046)

摘　　要：研究了50 Hz工频均匀强磁场(0.097 T)对人低分化胃腺癌细胞株—SNU细胞和人淋巴细胞胞浆钙离子浓度的影响.将体外培养的SNU细胞和人淋巴细胞,施加50 Hz工频均匀强磁场(0.097 T),处理不同时间后,应用共聚焦显微镜检测胞浆内钙离子浓度.实验表明,给予工频均匀强磁场处理后,SNU细胞和人淋巴细胞胞浆内钙离子浓度均升高.The study was designed to observe the effects of 50 Hz homogeneous ferromagnetic field （0.097 T） exposure on change of intracellular calcium-ion concentration of human low differentiation gastric adenocarcinoma cell line-SNU and lymphocytes in vitro. SNU cells and human peripheral blood lymphocytes in vitro were exposured to 50 Hz homogeneous ferromagnetic field at different time peroids. Cells were harvested after magnetic field exposure. The concentration of intracellular calcium-ion of cells in control and different experimental groups were measured with confocal microscope. The concentration of intracellular calcium-ion of SNU cells and human peripheral blood lymphocytes were all increased after magnetic field exposure.

关 键 词：工频均匀磁场 SNU细胞 人淋巴细胞 钙离子浓度 共聚焦显微镜 

分 类 号：Q26[生物学—细胞生物学] S968.431[农业科学—水产养殖]