TENS对关节炎大鼠脊髓三种神经肽含量的影响  

Effects of TENS on three neuropeptides in rats spinal cord with adjuvant mono-arthritis

作　　者：郝银菊[1] 蒋袁絮[1] 

机构地区：[1]宁夏医学院基础学院药理学教研室,宁夏银川750004

出　　处：《南京医科大学学报（自然科学版）》2008年第2期170-172,214,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：宁夏卫生厅重点课题项目(W200718)

摘　　要：目的:通过比较2Hz及100Hz跨皮神经电刺激(TENS)治疗大鼠慢性单发佐剂性关节炎症痛时三种神经肽含量的变化,探讨其在TENS治疗中的作用。方法:采用油包水型完全弗氏佐剂(CFA)右侧踝关节腔内注射致炎,TENS治疗,放射免疫分析法测定致炎前及致炎后1～9周大鼠脊髓背半侧组织中与疼痛有关的神经肽:八肽胆囊收缩素(CCK-8)、孤啡肽(OFQ)及内吗啡肽-2(EM2)含量的动态变化。结果:致炎后2～5周,CCK-8含量明显升高(P<0.05,P<0.01),100Hz TENS使关节炎时脊髓CCK-8含量升高的时间推迟至第3周且维持时间明显缩短;100Hz较2Hz TENS能更明显的升高炎症后期降低的脊髓OFQ含量;致炎后1～9周,关节炎大鼠脊髓EM2含量均明显下降(P<0.01),100Hz TENS使炎症所致EM2降低时间推迟至第2周,2Hz和100Hz TENS均可使炎症后期(5～9周)EM2含量恢复正常。结论:2Hz及100Hz TENS对慢性炎症痛大鼠脊髓组织中有关的神经肽,CCK-8、OFQ和EM2的影响可能是两者疗效存在差异的机制之一。Objective：To compare the effect of 2 Hz or 100 Hz transcutenous electrical nerve stimulation （TENS） on treating chronic pain with adjuvant monoarthritis in rats,and discuss changes of opioid peptide and anti-opioide peptide related to therapy effect.Methods：Water-in-oil type complete Freund＇s adjuvant （CFA） was injected into the right tibio-tarsal joint to produce a monoarthritic model. After treated with TENS,the content of CCK-8, OFQ and EM2 was measured by radio-immunoassay in spinal perfusate and dorsalhalf of the spinal cord. Results： ①2-5 weeks after arthritis, the content of CCK-8 in spinal cord was increased significantly （P 〈 0.05,P 〈 0.01）, 100 Hz TENS postponed peak time of CCK-8 from 2nd week to 3rd week. ②100 Hz TENS was more effective than 2 Hz TENS in increasing content of OFQ. ③1-9 weeks after arthritis,the content of EM2 in spinal cord was decreased significantly （P 〈 0.01）, 100 Hz TENS postponed peak time of EM2 to 2nd week,and both 2 Hz and 100 Hz TENS ean lead content of EM2 to normal during later period Of arthritis（5-9 weeks）. Conclusion：Changes of neuropeptide in spinal cord related to analgesia could take place in different degrees during arthritis,and different influence on opioide and antiopioid peptide might be involved in the mechanism of variation between two therapeutic effects of 2 Hz and 100 Hz TENS.

关 键 词：关节炎 慢性痛 跨皮神经电刺激 放射免疫分析法 神经肽 

分 类 号：R684[医药卫生—骨科学]