草莓红中柱根腐致病菌毒素提取及其活性测定  

Extraction and Bioassay of the Toxin Produced by Fusarium Solani

作　　者：穆丽松[1] 于慧瑛[2] 赵立庆[3] 刘志恒[1] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161 [2]铁岭林业局,辽宁铁岭112000 [3]铁岭农科院,辽宁铁岭112616

出　　处：《农业科技与装备》2008年第1期27-29,共3页Agricultural Science & Technology and Equipment

摘　　要：采用活性炭法、有机溶剂法和硫酸铵分级沉淀法3种方法提取毒素,并测定毒素活性。试验结果表明:3种方法均能提取到毒素,其中硫酸铵分级沉淀法是比较简便而相对有效的方法;经生物测定,该毒素能使草莓叶柄腐烂;经滤液透析稳定性测试,该毒素为大分子化合物,这与用有机溶剂提取毒素所得结果一致,同时测得其具有很强的热稳定性。In this article, three extraction methods of toxin from Fumarium solani were experimented, and fractionated precipitation with arrammonium sulfate was an effective and convenient method. Bioassay results indicated that the toxin can cause rot of strawberry stalk, and then the results of dialyzation showed that the toxin was a kind of big molecule compound with thermal stability in high temperature.

关 键 词：植物保护 草莓红中柱病菌 研究 致病毒素 提取 活性测定 

分 类 号：S432.44[农业科学—植物病理学]