慢性日本血吸虫感染C57BL/6小鼠体内Th1/Th2细胞凋亡的检测  

Detection of apoptosis of Th1 and Th2 cells in C57BL/6 mice chronically infected with Schistosoma japonicum

作　　者：许馨予[1] 赵嘉庆[1] 迟莹[1] 贺蕾[1] 温小云[1] 苏川[1] 

机构地区：[1]南京医科大学病原生物学系,南京210029

出　　处：《中国血吸虫病防治杂志》2008年第1期8-11,F0002,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(30571629)

摘　　要：目的观察日本血吸虫感染晚期C57BL/6小鼠体内Th1、Th2细胞凋亡水平的变化。方法运用流式细胞技术,采用表面分子、胞内因子同时染色的三色标记和间接标记的方法,对日本血吸虫感染13周的C57BL/6小鼠脾脏、淋巴结中的Th1、Th2细胞的凋亡水平进行观察。结果日本血吸虫感染晚期C57BL/6小鼠与正常小鼠相比,Th1、Th2细胞凋亡均显著增加,但Th1细胞凋亡较多而Th2细胞凋亡较少。此阶段Th1细胞凋亡比Th2细胞更加易感。结论Th1、Th2细胞凋亡易感性的不同可能是导致血吸虫感染晚期Th2极化的原因之一,此为血吸虫感染过程中Th极化研究提供了新的证据。Objective To observe the apoptosis of Th1 and Th2 cells in C57BL/6 mice chronically infected with Schistosoma japonicum. Methods The apoptotic Th1 and Th2 cells in spleen and lymph node from C57BL/6 mice infected with Schistosoma japonicum for 13 weeks were examined by three-color and indirect flow cytometery with staining surface molecule and intracellular cytokines. Results Compared with the normal mice, the proportion of apoptotic Th1 and Th2 cells of 13-week post-infection was significantly high, and the apoptotic Th1 cells increased more than apoptotic Th2 cells in the infected C57BL/6 mice, and the Th1 cells were more susceptible to apoptosis than Th2 cells. Conclusions Unequal susceptibility to apoptosis in Th1 and Th2 cells may be one of the reasons leading to Th2 polarization on mice chronically infected with Schistosoma japonicum, which provides the new proof of Th polarization.

关 键 词：日本血吸虫 TH细胞 凋亡 小鼠 

分 类 号：R383.24[医药卫生—医学寄生虫学]