重组日本血吸虫超氧化物歧化酶融合蛋白对小鼠免疫保护作用的研究  

Protective effect of recombinant cytosolic superoxide dismutase fusion protein of Schistosoma japonicum in immunized mice

作　　者：余传信[1] 李健[1] 殷旭仁[1] 吴宇迪[1] 华万全[1] 宋慧卓[1] 梁幼生[1] 高琪[1] 

机构地区：[1]江苏省血吸虫病防治研究所、卫生部寄生虫病预防与控制技术重点实验室、江苏省寄生虫分子生物学重点实验室、江苏省寄生虫病学重点学科,无锡214064

出　　处：《中国血吸虫病防治杂志》2008年第1期12-15,F0002,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(30471515、30671833);江苏省科技厅公益专项基金(BM2007704);江苏省医学重点人才基金(RC2007095)

摘　　要：目的探讨重组日本血吸虫胞浆内超氧化物歧化酶(SOD)融合蛋白在抗血吸虫感染中的免疫保护作用。方法采用亲和层析方法制备纯化表达的重组SOD融合蛋白。用重组SOD融合蛋白加福氏佐剂,免疫C57BL/6J小鼠,4周后用(45±2)条日本血吸虫尾蚴攻击感染,45d后剖杀小鼠,计算减虫率和减卵率,组织切片观察小鼠肝脏的病理变化。结果实验组减虫率为35.63%,减卵率为31.17%。实验组小鼠肝脏虫卵肉芽肿数比对照组少,单个肉芽肿的平均直径比对照组小22.32%,血清抗SOD融合蛋白特异性抗体亚类IgG1、IgG2a、IgG2b水平明显高于对照组(P均<0.05)。结论重组SOD分子能诱导一定水平的抗日本血吸虫感染免疫保护作用。Objective To explore the protective effect of recombinant superoxide dismutase （SOD） fusion protein against the infection of Schistosorna japonicum Chinese strain. Methods The recombinant SOD fusion protein was expressed and purified with Glutathione sepharose 413. C57BL/ 6J mice were immunized with the recombinant SOD fusion protein mixed with Freund adjuvant. Four weeks after the final immunization, the mice of the experiment and control groups were challenged with （45±2） S. japonicum cercariae. All the mice were sacrificed on the forty-fifth day after the challenge to calculate the worm reduction rate and egg reduction rate, and to observe the pathologic changes of liver tissue of the mice. Results The worm reduction rate was 35. 63% and the egg reduction rate was 31.17% in the experiment group. The number of granuloma in the live tissue of the experiment group was less than that of the control group, and the mean diameter of single granuloma in the experiment group reduced by 22.32% compared with that of the control group. The IgG subclass levels of IgG1, IgG2a, IgG2b were higher than those of the control group. Conclusion The recombinant SOD fusion protein has a protective effect against Schistosorna japonicum infection.

关 键 词：日本血吸虫 超氧化物歧化酶 免疫 保护作用 小鼠 

分 类 号：R383.24[医药卫生—医学寄生虫学]