薄层—质谱法测定绞股蓝皂甙A中的糖基顺序  

Determination on the Glycosyl Sequence of Gypenoside A by TLC-FABMS

作　　者：刘国樵[1] 郭端莱[1] 钟荣辉 姚仲平[2] 温汉辉[2] 何维德[2] 胡文梅[3] 

机构地区：[1]广州市医药工业研究所,广州510240 [2]中山大学分析测试中心,广州510275 [3]广东省医药学校,广州510520

出　　处：《中药材》1997年第8期398-399,共2页Journal of Chinese Medicinal Materials

摘　　要：用薄层—质谱法测定了绞股蓝皂甙XL Ⅲ中的糖及其顺序。方法快速、方便,可作为结构测试的手段。The glycosyl sequence of gypenoside A (A) isolated from Gynostemma pentaphyllum Makino was determinated by TLC-FABMS. The gypenoside A was hydrolysed with cellulase and the hydrolysate showed eight spots on a TLC plate, and the FABMS analysis of these spots indicated they corresponded to gypenoside A, A-Rham, A-Glu, A-Rham-Glu, A-Rham-Glu, A-Glu-Glu, A-Rham-2Glu, A-Rham-2Glu in order. Two chains can be determined to be Rham-Glu, and Glu-Glu, respectively. So the glycosyl sequence of gypenoside A was identical of gypenoside XLIII.

关 键 词：质谱法 绞股蓝皂甙 糖基 顺序 薄层扫描 

分 类 号：R284.1[医药卫生—中药学]