基于基因表达谱数据建立肾虚证量化数学模型  

Establishment of a Quantitative Mathematical Model of Shen-deficiency Syndrome Based on Whole-genome Transcriptional Profiles

作　　者：沈自尹[1] 张新民[1] 林伟[2] 吴斌[1] 黄建华[1] 刘小雨[1] 许世雄[3] 

机构地区：[1]复旦大学附属华山医院中西医结合研究所,上海200040 [2]复旦大学数学系 [3]复旦大学力学工程系

出　　处：《中国中西医结合杂志》2008年第2期131-134,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家重点基础研究计划973项目子课题(No.2007CB507400);国家自然科学基金(No.904090010);上海市科委重点基础研究计划(No.04JC14090)

摘　　要：目的利用基因表达谱数据,建立量化肾虚证程度的数学模型。方法采用4、10、18、24月龄SD大鼠,以24月龄大鼠加淫羊藿总黄酮(EF)干预,摘取大鼠下丘脑、垂体、肾上腺、淋巴细胞、骨、肝、肾、脾,利用基因芯片技术,检测全基因组mRNA表达,用不同月龄大鼠的基因表达谱数据建立神经网络模型,然后用此模型评价EF干预肾虚证的效果。结果7个组织共筛选到199个基因表达具年龄依赖特征,其中相当一部分为神经内分泌免疫相关基因。模型预判发现EF作用后,24月龄老龄大鼠下丘脑、垂体、肾上腺、肝、肾、骨、脾的基因表达与8～13月龄大鼠相似,分别为12.64、10.87、8.10、12.70、11.93、13.14、10.13月龄。结论基于基因表达谱数据能建立量化肾虚证程度的模型,并能预测补肾药的效果,研究也表明EF能使老年状态基因表达向年轻化靠近,提示其具有改善肾虚、延缓衰老的作用。Objective To establish a quantitative mathematical model of Shen-deficiency syndrome of TCM by utilizing whole-genome transcriptional profiles. Methods The 4, 10, 18, 24 months old SD rats were used, 24-months aged rats intervened by Epimedium Flavonoids （EF） were adopted in the experiment. Rats＇ hypothalamus, pituitary, adrenal, lymphocytes, bone, liver, and kidney, and spleen were taken for determining whole-genome mRNA expression with gene chip, and a quantitative nerve network model was established by utilizing the gene expression profile of different aged rats, then the model was used to evaluate the effects of EF on Shen-deficiency syndrome. Results Totally 199 genes showing age-dependent characteristics were screened out from the 7 kinds of tissue, most of them were neuro-endocrine immune related genes. Evaluation based on the mathematical model showed the age of hypothalamus, pituitary, adrenal, liver, kidney, bone, and spleen in the 24-months rats after EF intervention was 12.64, 10. 87, 8. 10, 12. 70, 11.93, 13.14, and 10. 13 months, respectively. Conclusion A quantitative mathematical model can be established based on the gene expression profile, it is suitable for estimating the efficacy of Shen-tonifying drugs. EF can make the gene expression of elder close to the young state, suggesting that EF has action in improving Shen-deficiency syndrome and delaying senescence.

关 键 词：肾虚证 基因表达谱 淫羊藿总黄酮 数学模型 

分 类 号：R256.5[医药卫生—中医内科学]