参芪扶正注射液对小鼠肝转移癌组织基因表达谱的影响  

Effects of Shenqi Fuzheng Injection on Gene Expression Profile of Liver Tissue with Metastatic Carcinoma in Mice

作　　者：丁治国[1] 李乃卿[1] 陶德胜 刘东来 杨扬[1] 史晓光[1] 邢光明[1] 魏永煜 李兰芳[3] 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]国家中药现代化工程技术研究中心 [3]中国中医科学院中药研究所肿瘤实验室

出　　处：《中国中西医结合杂志》2008年第2期135-138,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：教育部产学研综合项目基金资助(No.2006D90504018)

摘　　要：目的探讨参芪扶正注射液对小鼠肝转移癌组织基因表达谱的影响。方法20只小鼠采用将结肠癌C26细胞株瘤细胞液0.2mL注射于脾脏建立肝转移癌模型,随机分为两组,造模后5日,分别给予参芪扶正注射液(参芪组)和生理盐水(模型组)腹腔内注射,隔日1次,共5次;造模后15日处死动物留取检测标本,应用小鼠全基因芯片分析两组肝癌组织基因表达谱。结果(1)造模成功率100%;(2)基因表达:与模型组比较,参芪组上调基因123个,其中Ensemble数据库登录52个;下调基因1个,其中Ensemble数据库登录0个。结论参芪扶正注射液主要是通过上调多种基因促进肿瘤细胞凋亡和稳定染色体而发挥抗肿瘤作用。Objective To explore the effect of Shenqi Fuzheng Injection （SFI） on gene expression profile of liver tissue with metastatic carcinoma in mice. Methods Twenty liver metastatic carcinoma model mice were established by splenectomy after their spleens were injected with 0.2 mL colon cancer C26 cell strain oncocyte liquid, then they were randomly divided into the model group and the SFI group. Starting from the 5th day after modeling, mice in the model group and the SFI group were given via intraperitoneal injection once every other day with physiological saline and SFI respectively. All the mice were sacrificed at the 15th day and the gene profile of metastatic liver carcinoma tissue in the two groups were screened by whole genome chip technique. Results （1） The model establishing successful rate reached 100% ; （2） Gene expression showed that as compared with the model group, in the SFI group, 123 genes were up-regulated, with 52 of them registered to down-regulated and registered to Ensemble was none. Conclusion SFI plays Ensemble, while only one gene was its role of anti-tumor mainly by upregulating several relative genes to promote apoptosis of tumor ceils and stabilizing chromosomes.

关 键 词：参芪扶正注射液 基因表达谱 肝转移癌 

分 类 号：R285.5[医药卫生—中药学] R735.7[医药卫生—中医学]