昆明山海棠及其提取物对兔血管球囊损伤后内膜增生的抑制作用  

Extract from Tripterygium Hypoglaucum Hutch inhibits neointimal formation of rabbit carotid arteries after balloon angioplasty

作　　者：祖凌云[1] 喻卓[2] 郭艳红[1] 高炜[1] 

机构地区：[1]北京大学第三医院心内科,北京市100083 [2]昆明医学院第一附属医院心内科

出　　处：《中国介入心脏病学杂志》2008年第1期35-39,共5页Chinese Journal of Interventional Cardiology

摘　　要：目的探讨昆明山海棠(THH)及其提取物THW-4对兔血管球囊损伤后内膜增生的作用。方法建立兔颈动脉损伤模型,采用THH中药灌胃及THH中药灌胃/THW-4局部灌注两种给药方式,分别观察用药14d和28d后药物对血管新生内膜形成及内皮修复的影响。结果用药后14d,增殖细胞核抗原(PCNA)免疫组织化学染色显示,THH灌胃组和联合用药组新生内膜中PCNA染色阳性细胞比率均明显少于对照组(17.68%±5.27%,6.60%±2.52%比40.37%±7.12%,P<0.01);伊文思蓝活体染色显示各组内皮修复差异不明显。用药后28d血管壁定量组织形态学分析显示,THH灌胃组和联合用药组血管新生内膜面积及内膜面积与中膜面积比均显著低于对照组(2.95±0.65mm2,3.49±0.44mm2比5.25±0.53mm2;0.53±0.10,0.69±0.10比0.97±0.14,P均<0.01)。结论THH及THW-4可有效抑制血管球囊损伤后血管平滑肌细胞的增殖及新生内膜的形成。Objective To study the effects of Tripterygium Hypoglaucum Hutch （THH） and its extract （THW-4） on neointimal formation of rabbit carotid arteries after balloon angioplasty. Methods After injury of the common carotid arteries was set up by balloon angioplasty, the rabbits were divided into 3 groups and were given different treatment protocol including gastrogarage of THH （n = 9 ）, associated gastrogavage of THH with local administration of THW-4 into the injured arteries （n = 9 ） and 0. 25% DMSO saline local administration as the control （n = 9 ）. Neointimal fomation was evaluated on days after the procedure by proliferating all nuclear antigen （PCNA） and Evans Blue staining, and quantitative histomorphometric analysis was performed on 28 days. Results The PCNA-positive index in the neointima at day 14 after the procedure was reduced in the THH gastrogavage group and the THH/THW-4 group compared with the control （ 17.68% ± 5.27%, 6. 60%± 2. 52% vs 40. 37%± 7.12%, P 〈 0. 01 ）. The area of re-endothelialization evaluated by Evans Blue staining demonstrated no difference among the three groups at 14 days after the procedure. The intimal area and the intimal/medial ratio （I/M） at 28 days after the procedure were reduced in the THH gastrogavage group and the THH/THW-4 group compared with the control （2.95 ±0.65 mm^2, 3.49 ±0.44 mm^2 vs 5.25 ±0.53 mm^2; 0. 53 ±0. 10, 0.69 ±0. 10 vs 0.97 ± 0. 14, P 〈 0. 01 ）. Conclusion THH and THW-4 may inhibit VSMC proliferation and neointimal formation of injured rabbit carotid arteries after balloon angioplasty.

关 键 词：昆明山海棠 提取物 血管平滑肌细胞 血管内膜 

分 类 号：R543[医药卫生—心血管疾病] R282.71[医药卫生—内科学]