抑制素B βB亚单位在不同病理分型的无精子症患者睾丸组织中的表达  

Expression of Inhibin B βB Subunits in the Testicular Tissues of Azoospermia Patients with Different Pathological Alterations

作　　者：董志英[1] 于辉[1] 修贺明[1] 孙辉臣[1] 邱建宏[1] 

机构地区：[1]白求恩国际和平医院泌尿外科,河北石家庄050082

出　　处：《中华男科学杂志》2008年第1期20-22,共3页National Journal of Andrology

基　　金：河北省人口与计划生育委员会资助(2003-04)

摘　　要：目的:探讨抑制素B(INH B)βB亚单位在不同生精功能状态的人睾丸组织中的表达情况。方法:对83例无精子症患者进行睾丸组织病理检查诊断,根据病理形态的不同分为:唯支持细胞综合征型(n=21);生精功能低下型(n=20);生精阻滞型(n=24);生精功能基本正常型(n=18)。选择上述各型结构完整的睾丸组织,分别应用免疫组化法(SP)对血清INH B βB亚单位在不同生精功能状态的睾丸组织,进行定位研究。结果:各型睾丸组织内均存在血清INH B βB的表达,其分布特点为:间质细胞(Leydig cell)和早期生精细胞多为强阳性表达,呈深棕黄色;支持细胞(Sertoli cell)多为阳性表达;而晚期精子细胞和成熟精子未见表达;生精小管管周类肌细胞呈弱阳性表达。结论:INH B可能是睾丸Sertoli细胞和早期生精细胞的一个联合产物。Objective： To investigate the expression of inhibin B βB subunits in human testicular tissues. Methods： Eighty-three cases of the azoospermia underwent testicular biopsy. In accordance with the pathological alterations of spermatogenesis, the samples were divided into four groups： Sertoli-cell-only syndrome （ n = 21 ） ; hypospermatogenesis （ n = 20）, maturation arrest （ n = 24） and al-most normal spermatogenesis （n = 18）. Immunohistochemical staining for inhibin B βB subunits was conducted on the paraffin-embed- ded sections of different spermatogenesis to localize inhibin B βB subunits in the seminiferous tubules. Results： Immunohistochemi-cally, positive products of inhibin B βB subunits were found in both the seminiferous tubules and interstitial tissues of the testis as brown or yellow particles in the cytoplasm. Leydig cells and early intermediate spermatogenic cells showed a very strong positivity; Sertoli cells in the seminiferous tubules were mostly positive; peritubular myoid cells showed a weak positive staining; but no positive expression of inhibin B βB subunits was found in late spermatids and mature sperm. Conclusion ： Inhibin B may be produced by beth Sertoli cells and early spermatogenic cells in the seminiferous tubules.

关 键 词：无精子症 血清抑制素B 睾丸组织 免疫组织化学染色 

分 类 号：R698.2[医药卫生—泌尿科学] Q513.2[医药卫生—外科学]