甲磺酸伊马替尼抑制黑素细胞游走的实验研究  

Study on the Inhibition of Normal Human Epidermal Melanocyte Migration by Imatinib Mesylate

作　　者：夏建新[1] 占部和敬 吴珊[3] 王劲风[1] 古江增隆[2] 

机构地区：[1]吉林大学第二医院皮肤科,吉林长春130041 [2]日本九州大学医学部皮肤科,日本福冈 [3]吉林大学基础医学院病理生物学教研室,吉林长春130021

出　　处：《中国皮肤性病学杂志》2008年第1期1-3,共3页The Chinese Journal of Dermatovenereology

基　　金：吉林省科技厅基金资助项目(200505113);教育部归国人员基金项目

摘　　要：目的探讨甲磺酸伊马替尼对人黑素细胞(NHEM)游走的抑制作用。方法培养NHEM,用四甲基偶氮唑蓝(MTT)还原法测定甲磺酸伊马替尼对NHEM生长和分化的影响;使用侵袭小室测定甲磺酸伊马替尼抑制NHEM的游走情况;用蛋白印记法测定甲磺酸伊马替尼抑制c-kit磷酸化情况。结果甲磺酸伊马替尼0.1μmol/L,1.0μmol/L,5.0μmol/L能够抑制由SCF50ng/mL引起的NHEM游走,并能抑制c-kit的磷酸化。结论甲磺酸伊马替尼抑制SCF引起的NHEM游走及抑制c-kit的磷酸化,为其用于防治色素痣治疗后的复发奠定了理论基础。Objective To investigate the role of Imatinib mesylate on inhibition of normal human epidermal melanocyte （NHEM） migration. Methods The growth and the differentiation of NHEM were observed by MTT assay. Invasion-chamber was applied to determine the ability of NHEM＇s migration. Finally phosphorylation of c-kit in NHEM was studied by western blot. Resuits When the concentrations of Imatinib mesylate in culture medium were reach to 0.1 μmol/L, 1.0 μmol/L and 5.0 μmol/L respectively, Imatinib mesylate inhibited NHEM migration that was induced by SCF and Imatinib mesylate also inhibited phosphorylation of c-kit in NHEM. Conclusion Imatinib mesylate can inhibit NHEM migration that was induced by SCF and Imatinib mesylate can also inhibit phosphorylation of c-kit in NHEM. This suggests that Imatinib mesylate may be potential used for prevention of nevus spilus recurrence.

关 键 词：甲磺酸伊马替尼 游走 黑素细胞 

分 类 号：R965[医药卫生—药理学] R329.2[医药卫生—药学]