一种新型蚯蚓纤溶酶的研究  

Isolation and Properties of a Novel Fibrinolytic Enzyme from an Earth Worm

作　　者：胡文军[1] 傅庭治[1] 

机构地区：[1]南京大学生物科学与技术系,南京210093

出　　处：《中药材》1997年第2期78-81,共4页Journal of Chinese Medicinal Materials

摘　　要：采用加提取剂的匀浆、盐析、超滤及层析方法从一种环毛蚓中获得一种新型纤溶酶。此酶分子量为22000,为单链蛋白。它不仅有强烈的直接溶解血栓及人纤维蛋白活性,而且还有纤溶酶原激活作用;动物试验表明此酶的毒副作用很小。本方法获得的酶的活性、纯度及得率都很高。A novel fibrinolytic enzyme is isolated from one species of Pheretima by means of homogenizing,extracting with an extractive agent, precipitating with ammuonium sulfate, ultrafiltration and chromatography. The enzyme consists of a single chain with an M. W. of 22000. It can not only dissolve human thrombi and fibrin directly and strongly,hut also activate human plasmmogen. The enzyme shows little toxic and side effects in animal tests. The activity,purity and etraction-rate of the enzyme in this report are all very high.

关 键 词：环毛蚓 纤溶酶 纤溶作用 纤溶酶原激活 

分 类 号：R973.2[医药卫生—药品]