IL-2／IL-2R对单核来源树突状细胞的体外分化发育和功能的作用  

Effect of IL-2/IL-2R on the maturation, differentiation and biological function of the monocyte-derived dendritic cell in vitro

作　　者：黄勇[1] 朱一蓓[1] 夏瑜[1] 汤琳[1] 陈冰[1] 孙治平[1] 仲维学[1] 张学光[1] 

机构地区：[1]苏州大学生物技术研究所 江苏省临床免疫学重点实验室,215007

出　　处：《中华微生物学和免疫学杂志》2008年第1期61-64,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金重点项目（30330540）;国防科工委重点科研资助项目（委技函2003-44号）;国家自然科学基金面上项目（30700728）

摘　　要：目的分别比较抗人CD40单抗和肿瘤坏死因子（tumor necrosis factor，TNF）对单核来源树突状细胞（monocyte-derived dendritic cell，Mo-DC）体外诱导表达CD25（IL-2Rα）的作用，并进一步探讨了IL-2在Mo-DC分化成熟和功能介导中的生物学效应。方法采用联合粒细胞-巨噬细胞集落刺激因子（granulocyte-macrophage colony stimulating factor，GM-CSF）和IL-4体外诱导Mo-DC的培养方法，分别加入CIMO单抗和TNF-α诱导Mo-DC成熟；免疫荧光标记分析Mo-DC表型；ELISA法测定1干扰素（interferon-γ，IFN-γ）的分泌水平；^3H-TdR掺入法分析Mo-DC对自体T细胞的促增殖效应。结果（1）CIMO信号能有效诱导成熟Mo-DC上调表达CD25（IL-2Rα），而TNF-α则无此作用；（2）Mo-DC在CD40激发后，加入不同剂量的IL-2继续培养可促进Mo-DC对IFN-γ的分泌，同时^3H-TdR掺入法的结果也显示，在Mo-DC培养时加入IL-2可以增强Mo-DC对T细胞的促增殖作用。结论CIMO激发的Mo-DC上调表达CD25（IL-2Rα），外源性IL-2能促进Mo-DC分泌IFN-γ和介导T细胞的促增殖效应。Objective To evaluate the expressions of IL-2Rα by mature dendritic cell （ DC ） induced by tumor necrosis factor α（TNF-α） and agonistic monoclonal antibody（McAb） against CD40 and to investigate the biological effect of IL-2 on the function of monocyte-derived DC （ Mo-DC ）. Methods Mo-DC, induced by granulocyte-macrophage colony stimulating factor（GM-CSF） and IL-4 in vitro, was treated with anti-CD40 McAb and TNF-α to elicit maturation respectively. Phenotypic analysis of Mo-DC was done by flow cytometry（FCM）. ELISA was performed to assess the levels of interferon γ（IFN-γ） secreted by Mo- DC treated with different concentrations of IL-2 and 3 H-TdR was applied to examine the ability to stimulate T lymphocyte proliferation. Results CD25 （IL-2Rα） was up-regulatetedly expressed on Mo-DC induced by anti-CD40 McAb but not TNF-α. The secretion of IFN-T by Mo-DC was enhanced after being activated by CD40 and culturing together with different doses of IL-2. The results of ^3H-TdR assay indicated that the capability of DC to promote the proliferation of T lymphocytes was augmented by exogenous IL-2. Conclusion CD40 activation could up-regulate the CD2.5 （IL-2Rα） expression by Mo-DC and induce the nuclear NF-κB nuclear translocation appeared in the early stage and exogenous IL-2 could enhance the secretion of IFN-γ by Mo-DC and endow Mo-DC with enhancement of T cell proliferationin vitro.

关 键 词：树突状细胞 CD25 IL-2 

分 类 号：R686[医药卫生—骨科学]