缬沙坦对血管衰老中凋亡调控基因Bcl-2、Bax表达的影响  

The experimental study of valsartan on the expressions of Bcl-2 and Bax genes regulated with apoptosis in vascular aging

作　　者：单海燕[1] 白小涓[1] 陈香美[2] 

机构地区：[1]中国医科大学附属第一医院循环内科,辽宁沈阳110001 [2]解放军总医院肾病中心

出　　处：《中国老年学杂志》2008年第3期222-225,共4页Chinese Journal of Gerontology

基　　金：国家973重点基础研究发展规划项目资助(编号:G2007CB507405)

摘　　要：目的探讨缬沙坦对血管衰老中凋亡调控基因Bcl-2、Bax表达的影响。方法健康Wistar大鼠分为青年组、衰老组及缬沙坦组,测定血浆丙二醛(MDA)、超氧化物歧化酶(SOD)水平,同时采用恒速注入流体方法测定各组大鼠颈动脉血管的顺应性,利用免疫组织化学染色法、RT-PCR法和Western印迹法分析各组大鼠凋亡调控基因Bcl-2、Bax的mRNA及蛋白表达水平。结果与衰老组相比,缬沙坦组MDA浓度显著降低(P<0.05),SOD浓度显著升高(P<0.05),颈动脉血管的顺应性增高,其中弹性面积有显著性差异(P<0.05),Bcl-2的mRNA及蛋白表达水平明显增高(P<0.05),Bax的mRNA及蛋白表达水平降低(P<0.05)。结论血管衰老有其特征性生理改变,Bcl-2、Bax的mRNA及蛋白表达的失衡可能是血管衰老的重要分子机制之一,缬沙坦有一定的逆转血管衰老的作用。Objective To explore the role of Bcl-2 and Bax gene regulated apoptosis in vascular aging and its action mechanism. Methods The healthy rat were divided into young, aging and valsartan groups to analyze MDA and SOD level in plasma, and to measure the flexibihty of rat carotid segment by constant liquid injection. Immunohistochemistry, RT-PCR and Western-blot were used to analyze the expression of apoptosis-regulation genes Bcl-2 and Bax. Results MDA concentration in valsartan group evidently declined（P 〈 0. 05）, but SOD markedly ascended（P 〈0. 05） ; the carotid flexibility increased, especially flexibihty area were significantly different（P 〈0. 05）, mRNA and protein expression of Bcl-2 increased （ P 〈 0. 05 ）, but Bax mRNA and protein expressions decreased （ P 〈 0.05 ） compared to those of the aging group. Conclusions Vascular aging has its special physiologic function changes; one of its molecular mechanisms might be associated with the disequilibrium of Bcl-2 and Bax expressions. Valsartan could reverse vascular aging.

关 键 词：血管衰老 细胞凋亡 BCL-2 BAX 缬沙坦 

分 类 号：R339.38[医药卫生—人体生理学]