IFN-γ拮抗^60Coγ射线刺激肺成纤维细胞增殖的机制研究  

Mechanism of IFN-γ antagonizing pulmonary fibroblast proliferation stimulated by ^60 Co γ-rays

作　　者：刁瑞英[1] 宋良文[1] 王少霞[1] 尹纪业[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中华放射医学与防护杂志》2008年第1期13-16,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目（30570545）

摘　　要：目的探讨IFN-γ拮抗^60Coγ射线刺激人肺成纤维细胞（HLF）增殖的机制。方法应用MTT比色法，观察TGF-β1、受照后大鼠血清促HLF增殖的时效和量效关系及IFN-γ对增殖的影响；用ELISA检测受照后大鼠血清、肺组织TGF-β1水平及IFN-γ对照后大鼠血清促HLF合成TGF-β1的影响。结果TGF-β1有促HLF增殖的作用；受照后大鼠血清能促HLF增殖并合成TGF-β1；IFN-γ显著拮抗血清的上述作用；受照后1—4周大鼠血清和肺组织TGF-β1水平逐渐增加。结论TGF-β1参与γ射线刺激HLF增殖的作用，而IFN-γ在一定程度上抑制HLF合成TGF-β1，并拮抗其促HLF增殖的作用。Objective To explore the mechanism of IFN-γ antagonizing human pulmonary fibroblasts （HLF） proliferation stimulated by ^60Co T-rays. Methods The time- and dose-effect of HLF proliferation promoted by TGF-β1 and rat serum after radiation as well as effect of IFN-γ on proliferation were observed by MTT colorimetric methods. Content of TGF-β1 in rat serum and pulmonary tissues after irradiation as well as the effect of IFN-γ on rat serum after radiation promoting HLF to synthesize TGF-β1 were determined by ELISA. Results TGF-β1 played a role in promoting HLF proliferation. The sera of irradiated rats could promote HLF proliferation and TGF-β1 synthesis, but the effect was inhibited by IFN-γ significantly （ P 〈 0.05）. The level of TGF-β1 in rat serum and lung tissues gradually increased from 1 to 4 weeks after irradiation. Conclusions TGF-β1 is involved in the process of γ-rays stimulating HLF proliferation, while IFN-γ can inhibit HLF from synthesizing TGF-β1 and antagonize its role of promoting HLF proliferation.

关 键 词：人肺成纤维细胞 照射 增殖 TGF-Β1 IFN-Γ 

分 类 号：R818.7[医药卫生—放射医学]