当归对小鼠放射性肺损伤中NF-κB的影响  

Down- regulation of NF-κB DNA binding activity by Angelica Sinensis to ameliorate radiation-induced pulmonary injury in mouse model

作　　者：曹振[1] 谢丛华[1] 周福祥[1] 骆志国[1] 韩光[2] 周云峰[1] 

机构地区：[1]武汉大学中南医院肿瘤放化疗科,430071 [2]武汉大学肿瘤防治研究中心

出　　处：《中华放射医学与防护杂志》2008年第1期24-27,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目（30472263）;教育部博士点专项基金资助项目（20040486045）

摘　　要：目的研究小鼠放射性肺损伤中NF-κB活性的变化趋势，并探究当归对其干预及防治肺损伤的作用。方法实验小鼠分为生理盐水对照组、当归对照组、单纯照射组和当归干预组4组，于1、24、72h、1、2、4、8、16和24周9个时间点分别取肺组织检测。用HE和Masson染色评价组织病理变化和纤维化；用免疫组化判断NF-κB P65蛋白的定位和相对含量；用TransAM^TM ELISA方法测定NF-κB活性。结果小鼠模型的组织病理符合经典改变，4周起出现较明显间质性肺炎，8周最严重，16周开始肺间质内胶原纤维沉积并渐加重。NF-κB活性在照射后24h和8周呈现出双相的高峰。预防应用当归后，各时间点当归干预组肺组织的病理改变较同时间点单纯照射组明显要轻，“潜伏期”延长。结论在小鼠放射性肺损伤发展进程中，NF-κB活性呈现双相的升高，预防应用当归能下调这种升高并表现出明显的改善作用。Objective To study the trend of NF-κB binding activity during the course of radiationinduced pulmonary injury （ RPI）, and to evaluate the intervention effect of Angelica Sinensis on it. Methods Adult female C57BL/6J mice were randomly divided into 4 groups： blank control group （N group）, Angelica Sinensis control group （ A group）, irradiation group （ NX group） and irradiation group with Angelica Sinensis intervention （AX group）. All mice from the NX and AX groups underwent single fraction of 12 Gy γ-ray delivered to the whole thorax. All mice were intraperitoneally injected 25% Angelica Sinensis injection （20 ml/kg） or identical volume Normal Sediumdaily injection initiated 1 week before irradiation, lasted till 2 weeks after irradiation. Mice were sacrificed at designated time points （1, 24, 72 h, 1, 2, 4, 8, 16 and 24 weeks）, and the whole lungs were removed freshly. HE and Masson staining were performed to provide histopathologic evidence and to evaluate the collagen deposit situation respectively. The immunohistochemical staining of NF-κB P65 protein was performed to identify the location as well as the relative content of P65 protein. The DNA binding activity of NF-κB was detected by TransAM^TM ELISA assay. Results HE and Masson staining manifested that visible pathological alterations began at 2 weeks, typical interstitial pneumonitis were showed at 4 and 8 weeks, collagen deposition was visible from 16 weeks. The NF-κB binding activity detection and the immunohistochemical half-quantity analysis showed two-phase elevation, at 24 h and 8 weeks. Preventive application of Angelica Sinensis revealed prominently ameliorative effect for RPI, with pathological improvement, decreased staining and lower NF-κB binding activity in beth peaks. Conclusions During the development process of RPI, NF-κB binding activity shows two-phase elevation. Chinese medicine Angelica Sinensis can down-regulate the elevation, showing noted ameliorative effect.

关 键 词：放射性肺损伤 NF-ΚB 当归 

分 类 号：R285.5[医药卫生—中药学]