大鼠早期放射性脑损伤后少突胶质细胞内Tau蛋白表达的变化特征  

Alteration of Tau protein in rat oligodendrocytes after early radiation-induced brain injuries

作　　者：邓翀[1] 张泉[1] 田野[1] 

机构地区：[1]苏州大学附属第二医院放疗科,215004

出　　处：《中华放射医学与防护杂志》2008年第1期28-31,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目（30170287）;江苏省卫生厅重大科研课题（K2004067）;苏州大学医学发展基金资助项目（EE123504）

摘　　要：目的观察大鼠全脑照射条件下皮质下白质区少突胶质细胞内Tau蛋白在不同剂量和不同时间点的表达情况。方法分别以单次2、10和30Gy照射大鼠全脑后24h、1周和1个月时用免疫组织化学法观察少突胶质细胞内Tau-1和Tau-5的表达，并分析剂量、照射后时间的影响。结果照射后24h Tau—1的表达迅速升高达到峰值，1周后开始下降，其表达与照射剂量呈正相关，1个月后恢复照射前水平。Tau-5的表达各组在不同时间点表达差异无统计学意义（P〉0．05）。结论在大脑受电离辐射后早期，少突胶质细胞内Tau蛋白出现迅速的一过性去磷酸化，并且呈剂量依赖性。这提示Tau蛋白在早期放射性脑损伤的发生、发展过程中可能起到了一定的作用。Objective To observe the expression of Tau protein in oligodendrocytes within subcortical white matter at different doses and time-points after whole-brain irradiation. Methods A model of brain radiation-induced injury was established in rat after whole-brain irradiation with single dose of 4 MeV electron beam. The expression of Tau-1 and Tau-5 in oligodendrocytes within subcortical white matter was analyzed by immunohistochemistry at different time-points （24 hours, 1 week and 1 month） and different doses （2, 10 and 30 Gy ）. Results The expression of Tau-1 in oligodendrocytes reached the peak level in 24 hours, which was 1.5- 2 times as high as the normal group. It began to decrease after 1 week and returned to the normal level in 1 month. The expression levels of Tau-5 showed no difference at different time points （ P 〉 0.05 ）. Conclusions Tau protein in oligodendrocytes undergo rapid and temporal dephosphorylation after early radiation-induced brain injury in a dose-dependent manner. It is suggested that Tau protein might play an important role in the early stage of brain radiation injury.

关 键 词：放射性脑损伤 早期 少突胶质细胞 TAU蛋白 免疫组织化学 

分 类 号：R818[医药卫生—放射医学]