藤茶双氢杨梅树皮素对人肝癌BEL-7404细胞增殖的抑制作用  

Inhibition effect of ampelopsin on proliferation of BEL-7404 human liver cancer cell

作　　者：郑作文[1] 唐云丽[1] 张玉萌[1] 

机构地区：[1]广西中医学院,南宁530001

出　　处：《中国药物应用与监测》2008年第1期4-6,共3页Chinese Journal of Drug Application and Monitoring

基　　金：广西科学基金资助项目(桂科基0639052)

摘　　要：目的:探讨藤茶双氢杨梅树皮素(APS)的抗肿瘤作用。方法:以MTT法、生长曲线法、克隆形成法观察APS对人肝癌BEL-7404细胞的体外抑制作用。结果:MTT法中,APS对BEL-7404细胞的IC50为22.99μg/mL;细胞生长曲线法提示其对BEL-7404细胞生长有明显抑制作用;克隆形成法中,药物浓度在9.88μg/mL以上时抑制率为100%,药物浓度为6.58μg/mL时抑制率为69.65%。结论:APS对BEL-7404细胞的增殖有明显的抑制作用。Objective：To investigate the anti-tumor effect of ampelopsin in vitro.Methods：The growth inhibition was analyzed by MTT, cell colony and cell growth curve measurement in the liver carcinoma BEL-7404.Results：The ampelopsin could obviously inhibit the growth of cultured BEL-7404 cells, the IC50 in MTr assay is 22.99μg/mL; Ampelopsin could obviouly affect on the tumor cells＇growth; The repressive ratio in cell colony assay is 100% when medicine density is over 9.88μg/mL, the repressive ratio in cell colony assay is 69.65% when medicine density is 6.58μg/mL.Conclusion：Ampelopsin can inhibit the growth of BEL-7404 in vitro.

关 键 词：藤茶双氢杨梅树皮素 抗肿瘤 人肝癌BEL-7404细胞 

分 类 号：R285[医药卫生—中药学]