丙型肝炎病毒分片段抗体与HCVRNA的检测分析  

The detection and analysis of different region anti-bodies and HCVRNA of HCV

作　　者：贾艳会[1] 刘树业[2] 丁贤[2] 

机构地区：[1]天津医科大学,天津300070 [2]天津市第三中心医院,天津300170

出　　处：《哈尔滨医药》2008年第1期1-2,共2页Harbin Medical Journal

摘　　要：目的探讨丙型肝炎病毒(HCV)分片段抗体(HCV-C,HCV-NS3,HCV-NS4,HCV-NS5)对丙肝的诊断价值。方法对ELISA法抗-HCV总抗体阳性的55份阳性血清及80份可疑血清再进行分片段抗体ELISA检测及RT-PCR。结果55份ELISA法抗-HCV总抗体阳性血清经分片段抗体ELISA检测后,抗-NS3、抗-C的检出率最高,分别为96.4%、89.1%;两个以上片段检测结果阳性的血清有54份,与RT-PCR结果(HCVRNA阳性52份)高度符合。80份可疑血清中10份抗-C和抗-NS3同时阳性和9份HCVRNA阳性。结论HCV分片段抗体ELISA法有很高的敏感性和特异性,与RT-PCR法基本一致。抗-NS3、抗-C在HCV的诊断中有重要的意义,抗-NS5和抗-NS4有诊断的互补作用。Objective To discuss the diagnostic valhe of HCV different region anti - bodies for the diagnosis of HCV. Methods The different region anti - bodies and HCV - RNA were detected in 55 ELISA anti - HCV（ ＋ ） cases and 80 questionable cases. Results The positive rates of anti - NS3 and anti - C of the 55 samples were the highest, respectively 96.4% and 89.1%. There are 54 samples which has two or more positive regions, which was in accord with the HCV RNA assays （ there are 52 positive samples of HCV RNA ）. There are 10 samples with anti -C and anti - NS3 positive and 9 samples with HCV RNA positive. Conclusion Anti - HCV different Region ELISA has the same sensitivity and specificity as that of RT - PCR. Anti - NS3 and anti - C were very important to diagnose HCV, anti -NS5 and anti-NS4 had mutually complemented effect in diagnosis.

关 键 词：丙型肝炎病毒 分片段抗体 丙型肝炎病毒RNA 

分 类 号：R446.6[医药卫生—诊断学]