自体和异体骨髓间充质干细胞移植对家兔股骨头缺血性坏死治疗作用的比较研究  

Compared study of autografting and allografting of bone mrrow mesenchymal stem cells on avascular necrosis of femoral head in rabbits

作　　者：王江泳[1] 王保芝[1] 胡永成[2] 刘长安[3] 谈志龙[2] 王琳[4] 李会杰[4] 

机构地区：[1]河北医科大学人体解剖学教研室,石家庄050017 [2]天津医院骨肿瘤科 [3]白求恩国际和平医院骨科,石家庄 [4]河北医科大学第三医院骨病科,石家庄

出　　处：《中华骨科杂志》2008年第3期229-233,共5页Chinese Journal of Orthopaedics

基　　金：河北省科技厅科技攻关项目（No.2007276171）

摘　　要：目的比较同种异体和自体骨髓间充质干细胞（BMSCs）移植，对实验性家兔股骨头缺血性坏死的治疗作用；为建立BMSCs库及临床应用，提供实验证据。方法采用新西兰大白兔液氮冷冻法造模，随机分为对照组（A），髓芯减压组（B），自体细胞移植组（C）和同种异体细胞移植组（D）；对术后2、4、6、8周的标本，进行X线和组织学观察。结果（1）X线检查显示：2周时，A组股骨头标本呈高密度，随时间延长，骨密度进一步增加，并出现骨小梁结构的紊乱和囊性变，8周时变化最为显著。2周时，在B、C、D组股骨头钻孔区的边缘，出现密度增加；8周时，在B组股骨头钻孔区的周围，形成硬化线。在C、D组钻孔区，出现骨小梁结构。（2）组织学观察表明：液氮冷冻后，A组股骨头标本出现骨坏死和修复的病理变化。8周时B组钻孔区修复不完全。2周时C、D组钻孔区，均有大量移植的BMSCs存在；4周时两组钻孔区内，充满了新生骨小梁结构；在8周时钻孔区内，骨小梁趋于成熟。而且，C、D两组间骨小梁面积的图像分析，其差异无统计学意义。结论同种异体和自体BMSCs移植，对实验性家兔股骨头缺血性坏死的治疗，呈现相似的结果；建立BMSCs库供临床应用，具有较大的可行性。Objective To compare the repair effect of autografting and allografting of bone marrow mesenchymal stem cells （BMSCs） on avascular necrosis of femoral head （ANFH）in rabbits, and to supply experimental evidence for establishing BMSCs bank and clinical practice. Methods The 48 Newzland rabbits were divided into 4 groups randomly, as liquid nitrogen-freezing group （A）, decompression group （B）, BMSCs autografting group （C） and allografting group （D）. The animal model was made using freezing method with liquid nitrogen. The specimens from every group were observed with radioscopy and histology respectively at 2, 4, 6, 8 weeks after operation. Results （1）Radioscopic observation： After freezing by liquid nitrogen, the density of femoral head in group A increased gradually at 2, 4, 6, 8 weeks. In the meantime, trabecula became disorder and appeared cystic degeneration. The density around the drilled hole area （DHA） in group B, C, D increased at 2 weeks. At 8 weeks, the surrounding DHA presented low density and formed sclerotic line in group B, while more trabecula appeared in the DHA in group C, D. （2）Histological observation： The liquid nitrogen-freezing model in group A appeared the pathologic proceeding of ANFH. The repair effect was unsatisfaction in gronp B at 8 weeks. In group C, D, there were a large amount of BMSCs in the DHA at 2 weeks. And then DHA were filled of trabeculae at 4weeks. At 8 weeks, the matured trabeculae and marrow appeared in the intertrabecular space. There was no statistical difference between group C and D, by analyzing trabeculae area image. Conclusion There are resemble repair effect on rabbits ANFH with BMSCs autografting and allografting. It is feasible to establish BMSCs bank and present clinical apply.

关 键 词：股骨头坏死 细胞移植 移植 同种 骨髓祖代细胞 兔 

分 类 号：R681.8[医药卫生—骨科学]