过氧化氢对A549细胞转化生长因子β1和Smad3表达的影响  

Effects of hydrogen peroxide on the expression of transforming growth factor-β_1 and Smad3 in A549 cells

作　　者：杨冬梅[1] 丁宇[1] 应斌武[1] 蒋飞[1] 徐丹[1] 美朗曲措[1] 范红[1] 

机构地区：[1]四川大学华西医院呼吸内科,四川成都610041

出　　处：《中国呼吸与危重监护杂志》2008年第1期43-46,共4页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：国家自然科学基金资助项目(编号:30470761)

摘　　要：目的研究过氧化氢对A549细胞表达转化生长因子β1(TGF-β1)和Smad3磷酸化的影响。方法用过氧化氢建立体外A549细胞损伤模型,MTT法检测过氧化氢对A549细胞增殖活性的影响,免疫印迹法(Western Blotting)观察过氧化氢作用不同时间后A549细胞TGF-β1和Smad3磷酸化的表达。结果过氧化氢明显抑制A549细胞的增殖,浓度为1.0mmol/L时,其对A549细胞增殖的抑制率为46.34%;A549细胞在过氧化氢(1.0mmol/L)的刺激下,TGF-β1和Smad3磷酸化的表达量逐渐升高,24h时达到高峰,48h下降。结论氧化损伤早期,A549细胞表达TGF-β1和Smad3磷酸化增加,这可能与肺损伤后依赖Smad3的组织修复有关。Objective To observe the effects of hydrogen peroxide on the expression of transforming growth factor-β1 （TGF-β1）and Smad3 protein in A549 cells. Methods A549 cells were cultured with different concentrations of hydrogen peroxide. MTr assay was used to determine the cell growth and survival rates. The level of TGF-β1 and p-Smad3 protein were detected by western blotting. Results It was observed that hydrogen peroxide significantly inhibit proliferation of A549 cells. When the concentration of hydrogen peroxide was 1.0 mmol/L,the inhibition ratio reached 46. 34% ,and the level of TGF-β1 and p-Smad3 protein were increased in a time-dependence manner and reached a peak after 24 h, then decreased a little but also remained at high level. Conclusions In the early oxidative damage,A549 cells express high level of TGF-β1 and p-Smad3 protein. It may be relevant to tissue repair and remodeling after lung injury.

关 键 词：过氧化氢 转化生长因子Β1 SMAD3 

分 类 号：R563.8[医药卫生—呼吸系统]