应激对大鼠部分组织器官铁含量的影响  

EFFECT OF STRESS ON IRON IN SERUM,LIVER,SPLEEN,AND BONE MARROW OF RATS

作　　者：魏春岚[1] 黄雪强[2] 周健[1] 陈建波[1] 李敏[1] 

机构地区：[1]第二军医大学航海医学系军队卫生学教研室,上海200433 [2]上海长海医院中医科

出　　处：《解放军预防医学杂志》2008年第1期14-17,共4页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：国家自然科学基金项目课题(No.30471463);达能营养中心膳食营养研究与宣教基金研究项目课题

摘　　要：目的观察应激条件下大鼠血清、肝、脾和骨髓铁含量的变化。方法将SD大鼠随机分为空白对照组、心理应激组和足底电击组。实验应激周期分别为3d、7d和14d,各组应激结束后,处死动物,采用原子吸收法测定血清铁、肝铁和脾铁;采用酸性亚铁氰化钾染色法测定骨髓铁含量。结果与对照组相比,3d、7d和14d心理应激组大鼠的血清铁分别下降28.4%、27.7%和37.0%(P<0.05);而肝铁含量分别升高20.6%,41.3%和24.9%(P<0.05);脾铁分别升高4.38%(P>0.05)、12.3%和10.4%(P<0.05)。足底电击组血清、肝、脾铁含量与心理应激组变化趋势相同;7d、14d心理应激组和足底电击组大鼠骨髓铁含量均明显减少。结论心理应激同躯体应激(足底电击),均可导致大鼠血清铁降低;应激条件下大鼠体内铁发生重新分布,表现为血清铁和骨髓铁下降以及肝铁和脾铁升高。Objective To observe and evaluate the effects of foot-shock stress and psychological stress on iron in serum, liver, spleen, and bone marrow of rats. Methods SD rats were randomly divided into three groups： control group （CG）, foot-shock stress group （FSG）, and psychological stress group （PSG）. They were stressed for three periods （3 d, 7 d and 14 d）. At the end of the experiments,the amount of iron in serum,liver,and spleen was detected by atom absorption method and the amount of iron in the bone marrow was measured by staining of acidic potassium ferrocyanide. Results The serum iron decreased 28.4%, 27.7% and 37.0% more respectively（ P 〈 0.05）, while liver iron increased 20.6 %, 41.3 %, and 24.9 % more respectively （ P 〈 0.05 ）, spleen iron increased 4.38 % （ P 〉 0.05 ）, 12.3 %, and 10.4% more respectively（ P 〈 0.05）in PSG than in CG after 3 d, 7 d and 14 d of stress. The tendency of changes of serum and liver iron in FSG was similar to those of PSG. The bone marrow iron was decreased significantly in both PSG and FSG after 7 d and 14 d of stress. Conclusion Both psychological stress and physical stress （foot-shock stress） can result in a significant decrease in serum iron. There is a redistribution of iron in the body, resulting in decrease in serum and bone marrow iron, and increase in liver and spleen iron.

关 键 词：应激 血清铁 肝铁 脾铁 骨髓铁 

分 类 号：R395.1[哲学宗教—心理学]