无蹼壁虎视网膜GFAP免疫阳性的分布和衰老相关性变化  

The Distribution and Senescence-related Changes of GFAP-immunoreactivity in the Retina of Lizard Gekko swinhonis

作　　者：王智超[1] 王笑蕾[2] 武军元[3] 余中宾[1] 鲁亚平[1] 

机构地区：[1]安徽师范大学生命科学学院,芜湖241000 [2]泰山医学院内科护理教研室,泰安271000 [3]新疆生产建设兵团塔里木畜牧科技重点实验室塔里木大学动物科技学院,新疆阿拉尔843300

出　　处：《动物学杂志》2008年第1期16-20,共5页Chinese Journal of Zoology

基　　金：国家自然科学基金项目(No.30470537);安徽省教育厅自然科学基金重点项目(No.2005kj028zd)

摘　　要：运用免疫组织化学方法(ABC法)结合数学形态学技术观察了胶质纤维酸性蛋白(GFAP)在无蹼壁虎(Gekko swinhonis)视网膜的分布及与衰老相关性变化。结果显示,GFAP免疫阳性结果主要位于视网膜的Müller细胞内侧端的胞体、突起和膨大的终足,胞体外侧端呈弱阳性。比较成年组与老年组视网膜中央区GFAP免疫阳性形态学参数显示:老年组GFAP免疫阳性Müller细胞短径较成年组增粗;老年组GFAP免疫阳性细胞的光密度值较成年组增高;老年组视网膜各层GFAP免疫阳性面积占视网膜总面积的百分率较成年组增高。GFAP在无蹼壁虎视网膜的分布特点及年龄相关性变化,提示Müller细胞可能通过增加GFAP表达或减弱分解来参与视网膜衰老过程中的生理功能调节。Immunohistochemical and morphometric methods were used in the present study to investigate the distribution and senescence-related changes of GFAP-immunoreactivity（IR）in the retina of lizard Gekko swinhonis.The results showed that the GFAP-IR was mainly localized in the vitread endfeet and inner part of the radial trunks of the Müller cells through tout the retina.Compared with adult lizards,the old lizards had a significant increase in the optical density（OD）and the percentage of GFAP-IR in the retinas.At the same time,the GFAP-IR Müller cells in the old lizards showed shorter minor diameters.All the results suggest that Müller cells probably participate in the senescencing processes of the retina by the mediation of the higher GFAP expression and lower decompositon.

关 键 词：胶质纤维酸性蛋白 无蹼壁虎 年龄 分布 免疫组织化学 

分 类 号：Q954.6[生物学—动物学]